State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China.
Department of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, China.
Colloids Surf B Biointerfaces. 2021 May;201:111630. doi: 10.1016/j.colsurfb.2021.111630. Epub 2021 Feb 18.
Advances in nanotheranostics have promoted the development of precision medicine, which has great potential as a weapon for clinical diagnosis and therapy of tumors. However, the combination of three functional principle components (imaging probes, therapeutic agents and surface coating) in traditional theranostic system is difficult to be achieved in only one step, while undergoing multiple synthesis procedures, time-consuming process and unknown toxicity. Herein, we fabricated iodinated polyaniline (LC@I-PANi) nanoparticles via a facile one-step synthesis approach integrating chemical oxidative polymerization and iodine-doping process for computed tomography (CT) imaging and photoacoustic (PA) imaging-guided photothermal therapy (PTT). Iodic acid (HIO) as an oxidant induces chemical oxidation polymerization of aniline monomers. Meanwhile, iodine is incorporated into the polyaniline structural units in the process of polymerization to obtain LC@I-PANi nanoparticles. Moreover, thel-cysteine (LC) has an effect on diameter of LC@I-PANi nanoparticles, which enables nanoparticles have size-controlled spherical morphology and good colloidal stability. The hemolysis assay and cytotoxicity assessment verified the good biocompatibility of LC@I-PANi. Moreover, our LC@I-PANi nanoparticles could not only exhibit appealing PTT efficiency, but also achieve excellent CT/PA dual-mode imaging effect. The histological evaluations suggested the negligible toxicity of LC@I-PANi in vivo. This is the first time to our knowledge that multifunctional LC@I-PANi nanoparticles were prepared by an ingenious one-step method. This work not only highlights a one-step strategy that simplified the complex synthesis of LC@I-PANi nanoparticles, but also provides insight for further biomedical application of "all-in-one" theranostic agent.
纳米诊疗学的进展推动了精准医学的发展，它作为肿瘤临床诊断和治疗的有力工具具有很大的潜力。然而，传统的诊疗系统中的三种功能原理组件（成像探针、治疗剂和表面涂层）的结合很难在一步中实现，同时需要经过多次合成步骤，耗时且未知的毒性。在这里，我们通过一种简便的一步法合成方法，将化学氧化聚合和碘掺杂过程集成在一起，制备了碘化聚苯胺（LC@I-PANi）纳米粒子，用于计算机断层扫描（CT）成像和光声（PA）成像引导光热治疗（PTT）。碘酸（HIO）作为氧化剂诱导苯胺单体的化学氧化聚合。同时，在聚合过程中，碘掺入聚苯胺结构单元中，得到 LC@I-PANi 纳米粒子。此外，半胱氨酸（LC）对 LC@I-PANi 纳米粒子的直径有影响，使纳米粒子具有尺寸可控的球形形态和良好的胶体稳定性。溶血试验和细胞毒性评估验证了 LC@I-PANi 的良好生物相容性。此外，我们的 LC@I-PANi 纳米粒子不仅表现出令人满意的 PTT 效率，而且还实现了出色的 CT/PA 双模成像效果。组织学评价表明 LC@I-PANi 在体内具有可忽略的毒性。据我们所知，这是首次通过巧妙的一步法制备多功能 LC@I-PANi 纳米粒子。这项工作不仅突出了一种简化 LC@I-PANi 纳米粒子复杂合成的一步策略，而且为进一步将“一体化”诊疗剂用于生物医学应用提供了思路。
