Marques E S, Tsuboy M S F, Carvalho J C T, Rosa P C P, Perazzo F F, Gaivão I O M, Maistro E L
Programa de Pós-Graduação em Biologia Geral e Aplicada, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP, Brasil.
Departamento de Biotecnologia, Faculdade de Ciências e Letras, Universidade Estadual Paulista, Assis, SP, Brasil.
Genet Mol Res. 2017 Aug 17;16(3):gmr-16-03-gmr.16039700. doi: 10.4238/gmr16039700.
Euterpe oleracea Mart., popularly known as "açaí", is a tropical fruit from the Amazon region where it has considerable economic importance. Açaí has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety of using this fruit oil exclusively. Therefore, we evaluated the in vitro cytotoxic, genotoxic, and antigenotoxic effects of E. oleracea fruit oil (EOO) in cultured human lymphocytes (non-metabolizing cells) and HepG2 cell line (human hepatoma) (metabolizing cells) by using MTT, comet, and micronucleus assays. A wide range of EOO concentrations was tested with a preliminary MTT assay, which allowed selecting five concentrations for comet and micronucleus assays: 2.5, 10, 100, 500, and 1000 µg/mL. The results showed that none of the EOO tested concentrations presented cytotoxic effects. The genotoxic assessment revealed an absence of significant DNA and chromosome damage in human lymphocytes and HepG2 cells but did not show chemoprotection against the DNA damage induced by methyl methanesulfonate and benzo[a]pyrene, used as DNA-damaging agents.
Euterpe oleracea Mart.，俗称“阿萨伊果”，是一种来自亚马逊地区的热带水果，在当地具有相当重要的经济价值。阿萨伊果一直被用作食物和多种药用。尽管这种水果被广泛使用，但关于单独使用这种果油安全性的数据却很缺乏。因此，我们通过MTT法、彗星试验和微核试验，评估了E. oleracea果油（EOO）对培养的人淋巴细胞（非代谢细胞）和HepG2细胞系（人肝癌细胞）（代谢细胞）的体外细胞毒性、遗传毒性和抗遗传毒性作用。通过初步的MTT试验测试了一系列广泛的EOO浓度，这使得能够选择五个浓度用于彗星试验和微核试验：2.5、10、100、500和l000µg/mL。结果表明，所测试的EOO浓度均未呈现细胞毒性作用。遗传毒性评估显示，在人淋巴细胞和HepG2细胞中未出现显著的DNA和染色体损伤，但对用作DNA损伤剂的甲磺酸甲酯和苯并[a]芘诱导的DNA损伤未显示出化学保护作用。
