Health Sciences Faculty, University Vale do Rio Doce, Brazil; Integrated Pharmacology and Drug Interactions Research Group (GPqFAR), MG, Brazil.
Faculty of Pharmacy, Federal University of Minas Gerais, Brazil; Integrated Pharmacology and Drug Interactions Research Group (GPqFAR), MG, Brazil.
Microb Pathog. 2018 Jan;114:29-35. doi: 10.1016/j.micpath.2017.11.006. Epub 2017 Nov 13.
Euterpe oleracea (Açaí) fruit are widely consumed at the Brazilian Amazon region, and biological potentials such as immunomodulatory and antioxidant have been described for its extracts. However, its antimicrobial properties remain poorly investigated. Here, the antimicrobial and antibiofilm activities of the methanolic extract of an artisanally-manufactured açaí pulp (MEAP) were evaluated against clinical isolates of Staphylococcus aureus. Besides, MEAP interference on the activity of antimicrobial drugs of clinical relevance was explored, and its cytotoxicity against hepatocellular carcinoma cells (HepG2) was investigated. Biochemical and physicochemical properties of the pulp were investigated, and the presence of polyphenols on the extract was confirmed. For the first time, we report that the methanolic extract of açaí pulp is effective against planktonic cells and biofilms of S. aureus, and also decreased the proliferation of HepG2 cells. Statistically significant synergism was observed when the extract was combined to the tested antimicrobials except for erythromycin, and all biochemical and physicochemical parameters ranged within the accepted values established by the Brazilian legislation. Our data open doors for more studies on the antimicrobial activity of phytomolecules isolated from Euterpe oleracea extracts, and also for its combined use with antimicrobial drugs.
巴西亚马逊地区广泛食用西番莲果，其提取物具有免疫调节和抗氧化等生物潜能。然而，其抗菌性能仍未得到充分研究。本研究评估了一种手工制作的西番莲果浆的甲醇提取物（MEAP）对金黄色葡萄球菌临床分离株的抗菌和抗生物膜活性。此外，还研究了 MEAP 对临床相关抗菌药物活性的干扰作用，并检测了其对肝癌细胞（HepG2）的细胞毒性。对果肉的生化和物理化学性质进行了研究，并证实了提取物中存在多酚。这是首次报道西番莲果浆的甲醇提取物对金黄色葡萄球菌浮游细胞和生物膜具有活性，并且还能降低 HepG2 细胞的增殖。当提取物与测试的抗菌药物联合使用时，除了红霉素外，均观察到统计学上显著的协同作用，并且所有生化和物理化学参数均在巴西法规规定的可接受范围内。我们的数据为研究从西番莲果提取物中分离的植物化合物的抗菌活性以及与抗菌药物联合使用开辟了道路。
