与Seeplex RV15 OneStep ACE和PneumoBacter ACE相比，评估Anyplex™ II RV16和RB5实时逆转录聚合酶链反应用于同时检测上呼吸道病原体的情况。
Evaluation of Anyplex™ II RV16 and RB5 real-time RT-PCR compared to Seeplex RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens.
作者信息
Parrott Gretchen, Kinjo Takeshi, Nabeya Daijiro, Uehara Ayako, Nahar Saifun, Miyagi Kazuya, Haranaga Shusaku, Tateyama Masao, Fujita Jiro
机构信息
Department of Infectious Diseases, Respiratory and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Japan.
Department of Infectious Diseases, Respiratory and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Japan.
出版信息
J Infect Chemother. 2017 Dec;23(12):859-861. doi: 10.1016/j.jiac.2017.07.013. Epub 2017 Aug 19.
Abstract
This prospective study was performed to evaluate and compare the performance of the multiplex PCR Seeplex assays and Anyplex™ II assays. From May 2014 until April 2016, a total of 247 respiratory samples were collected in Okinawa, Japan. Multiple respiratory pathogens were detected in 37% of patients with positive results. The most prevalent pathogens were influenza A virus and respiratory syncytial virus B. Despite minor differences in capabilities, both the Seeplex assays and Anyplex™ II assays can be easily implemented in diagnostic or research laboratories to optimize the detection and management of respiratory pathogen induced diseases.
摘要
本前瞻性研究旨在评估和比较多重PCR Seeplex检测法和Anyplex™ II检测法的性能。2014年5月至2016年4月期间，在日本冲绳共采集了247份呼吸道样本。37%检测结果呈阳性的患者中检测到多种呼吸道病原体。最常见的病原体是甲型流感病毒和呼吸道合胞病毒B型。尽管在功能上存在细微差异，但Seeplex检测法和Anyplex™ II检测法都可以在诊断或研究实验室中轻松实施，以优化对呼吸道病原体所致疾病的检测和管理。
相似文献
1
Evaluation of Anyplex™ II RV16 and RB5 real-time RT-PCR compared to Seeplex RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens.与Seeplex RV15 OneStep ACE和PneumoBacter ACE相比，评估Anyplex™ II RV16和RB5实时逆转录聚合酶链反应用于同时检测上呼吸道病原体的情况。
J Infect Chemother. 2017 Dec;23(12):859-861. doi: 10.1016/j.jiac.2017.07.013. Epub 2017 Aug 19.
2
Evaluation of a novel real-time RT-PCR using TOCE technology compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses.评价一种新型的基于 TOCE 技术的实时 RT-PCR 与培养和 Seeplex RV15 联合检测用于同时检测呼吸道病毒的方法。
J Clin Virol. 2013 Aug;57(4):338-42. doi: 10.1016/j.jcv.2013.04.014. Epub 2013 Jun 3.
3
Comparison of Anyplex II RV16 with the xTAG respiratory viral panel and Seeplex RV15 for detection of respiratory viruses.Anyplex II RV16 与 xTAG 呼吸道病毒 panel 及 Seeplex RV15 检测呼吸道病毒的比较。
J Clin Microbiol. 2013 Apr;51(4):1137-41. doi: 10.1128/JCM.02958-12. Epub 2013 Jan 30.
4
Evaluation of the AdvanSure™ real-time RT-PCR compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses.将AdvanSure™实时逆转录聚合酶链反应与培养法和Seeplex RV15进行比较，用于同时检测呼吸道病毒的评估。
Diagn Microbiol Infect Dis. 2014 May;79(1):14-8. doi: 10.1016/j.diagmicrobio.2014.01.016. Epub 2014 Jan 30.
5
Comparative Clinical Evaluation of NeoPlex RB-8 with Seeplex PneumoBacter ACE for Simultaneous Detection of Eight Respiratory Bacterial Pathogens.NeoPlex RB-8 与 Seeplex PneumoBacter ACE 用于同时检测八种呼吸道细菌病原体的临床比较评估。
J Clin Microbiol. 2020 Jan 28;58(2). doi: 10.1128/JCM.01500-19.
6
Evaluation of three commercial multiplex assays for the detection of respiratory viral infections.三种用于检测呼吸道病毒感染的商业多重检测方法的评估。
J Virol Methods. 2017 Oct;248:39-43. doi: 10.1016/j.jviromet.2017.06.006. Epub 2017 Jun 17.
7
Comparison of the Anyplex(TM) II RV16 and Seeplex(®) RV12 ACE assays for the detection of respiratory viruses.用于检测呼吸道病毒的Anyplex™ II RV16和Seeplex® RV12 ACE检测方法的比较
Diagn Microbiol Infect Dis. 2014 Aug;79(4):419-21. doi: 10.1016/j.diagmicrobio.2014.01.025. Epub 2014 Feb 20.
8
Comparison of two multiplex PCR assays for the detection of respiratory viral infections.两种用于检测呼吸道病毒感染的多重PCR检测方法的比较。
Clin Respir J. 2014 Oct;8(4):391-6. doi: 10.1111/crj.12083. Epub 2014 Jan 10.
9
Comparison of the AdvanSure™ real-time RT-PCR and Seeplex(®) RV12 ACE assay for the detection of respiratory viruses.用于检测呼吸道病毒的AdvanSure™实时逆转录聚合酶链反应与Seeplex(®) RV12 ACE检测法的比较
J Virol Methods. 2015 Nov;224:42-6. doi: 10.1016/j.jviromet.2015.08.003. Epub 2015 Aug 13.
10
[Simultaneous detection of respiratory viruses and influenza A virus subtypes using multiplex PCR].[使用多重聚合酶链反应同时检测呼吸道病毒和甲型流感病毒亚型]
Mikrobiyol Bul. 2014 Oct;48(4):652-60. doi: 10.5578/mb.8221.
引用本文的文献
1
Characteristics of the Epidemic from 2019 to 2020 in Korea: Macrolide Resistance and Co-Infection Trends.2019年至2020年韩国疫情特征：大环内酯类耐药性及合并感染趋势
Antibiotics (Basel). 2023 Nov 13;12(11):1623. doi: 10.3390/antibiotics12111623.
2
Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults.在国家流感监测中，评估赛沛 RV15 多重 PCR 检测方法对住院成年患者中甲型流感亚型和乙型流感谱系的检测效果。
J Med Microbiol. 2020 Feb;69(2):256-264. doi: 10.1099/jmm.0.001032. Epub 2019 Jun 28.
3
Viral and Bacterial Etiology of Acute Febrile Respiratory Syndrome among Patients in Qinghai, China.中国青海急性发热呼吸道综合征患者的病毒和细菌病因。
Biomed Environ Sci. 2019 Jun;32(6):438-445. doi: 10.3967/bes2019.058.
本文引用的文献
1
Comparison of the Anyplex(TM) II RV16 and Seeplex(®) RV12 ACE assays for the detection of respiratory viruses.用于检测呼吸道病毒的Anyplex™ II RV16和Seeplex® RV12 ACE检测方法的比较
Diagn Microbiol Infect Dis. 2014 Aug;79(4):419-21. doi: 10.1016/j.diagmicrobio.2014.01.025. Epub 2014 Feb 20.
2
Evaluation of the AdvanSure™ real-time RT-PCR compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses.将AdvanSure™实时逆转录聚合酶链反应与培养法和Seeplex RV15进行比较，用于同时检测呼吸道病毒的评估。
Diagn Microbiol Infect Dis. 2014 May;79(1):14-8. doi: 10.1016/j.diagmicrobio.2014.01.016. Epub 2014 Jan 30.
3
Detection of respiratory viruses using a multiplex real-time PCR assay in Germany, 2009/10.2009/10年在德国使用多重实时聚合酶链反应检测呼吸道病毒
Arch Virol. 2014 Apr;159(4):669-76. doi: 10.1007/s00705-013-1876-3. Epub 2013 Oct 15.
4
Comparison of four multiplex PCR assays for the detection of viral pathogens in respiratory specimens.四种多重 PCR 检测方法在呼吸道标本中检测病毒病原体的比较。
J Virol Methods. 2013 Aug;191(2):118-21. doi: 10.1016/j.jviromet.2013.04.005. Epub 2013 Apr 11.
5
Comparison of Anyplex II RV16 with the xTAG respiratory viral panel and Seeplex RV15 for detection of respiratory viruses.Anyplex II RV16 与 xTAG 呼吸道病毒 panel 及 Seeplex RV15 检测呼吸道病毒的比较。
J Clin Microbiol. 2013 Apr;51(4):1137-41. doi: 10.1128/JCM.02958-12. Epub 2013 Jan 30.
6
Comparative evaluation of the Seegene Seeplex RV15 and real-time PCR for respiratory virus detection.比较赛沛 Seeplex RV15 与实时 PCR 检测呼吸道病毒的效果。
J Med Virol. 2011 Aug;83(8):1469-75. doi: 10.1002/jmv.22125.
7
Multiplex PCR and emerging technologies for the detection of respiratory pathogens.多重 PCR 和新兴技术在呼吸道病原体检测中的应用。
Clin Infect Dis. 2011 May;52 Suppl 4(Suppl 4):S326-30. doi: 10.1093/cid/cir047.
8
Utilization of nucleic acid amplification assays for the detection of respiratory viruses.利用核酸扩增检测法检测呼吸道病毒。
Clin Lab Med. 2009 Dec;29(4):661-71. doi: 10.1016/j.cll.2009.07.008.
9
Detection of respiratory viruses by molecular methods.通过分子方法检测呼吸道病毒。
Clin Microbiol Rev. 2008 Oct;21(4):716-47. doi: 10.1128/CMR.00037-07.
Zotero 插件