Key Laboratory of Aquatic Genomics, Ministry of Agriculture, Research Centre for Aquatic Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, China.
Department of Biological Science and Technology, Heilongjiang Vocational College for Nationalities, Harbin 150066, China.
Genomics. 2018 Jan;110(1):59-66. doi: 10.1016/j.ygeno.2017.08.006. Epub 2017 Aug 19.
For genetic linkage analysis of Japanese flounder, 160 doubled haploids (DH) were artificially produced using mitotic gynogenesis and were genotyped for 458 simple sequence repeat (SSR) markers, 101 of which show distortional segregation. The genetic linkage map was constructed by modifying recombination fractions between the distorted markers. Between the corrected and uncorrected genetic maps, there were considerable differences in genetic distance, but not in relative locations among markers. Using a liability model, a segregation distortion locus (SDL), with an additive genetic effect of 1.772, was mapped between markers BDHYP387 and Poli56TUF of chromosome 24 in the corrected genetic map. Additionally, six pairs of epistatic SDLs were identified on chromosomes 1, 5, 8, 9, 23, and 24. Changes in genetic distances between markers did not occur on chromosome regions with main effect SDLs. However, most chromosome regions where genetic distances changed covered the detected epistatic SDLs. This study concluded that epistatic SDLs decrease linkages between markers and lengthen genetic distances in Japanese flounder. This finding has been partially validated in other DH populations derived from three female Japanese flounders.
为了对牙鲆进行遗传连锁分析，我们采用有丝分裂雌核发育技术人工产生了 160 个加倍单倍体（DH），并对 458 个简单重复序列（SSR）标记进行了基因型分析，其中 101 个标记显示出偏分离。通过修正偏分离标记之间的重组分数，构建了遗传连锁图谱。在修正和未修正的遗传图谱之间，遗传距离存在较大差异，但标记之间的相对位置没有差异。利用易患性模型，在修正遗传图谱中第 24 号染色体上标记 BDHYP387 和 Poli56TUF 之间定位到一个加性遗传效应为 1.772 的分离扭曲位点（SDL）。此外，在第 1、5、8、9、23 和 24 号染色体上还鉴定到了 6 对上位性 SDLs。主效 SDL 所在的染色体区域的标记间遗传距离没有变化。然而，遗传距离变化的大多数染色体区域都覆盖了检测到的上位性 SDLs。本研究得出结论，上位性 SDLs 降低了牙鲆标记之间的连锁关系，延长了遗传距离。这一发现已经在来自 3 只雌牙鲆的其他 DH 群体中得到部分验证。
