Ari1327 矮秆基因 QTL 定位，Ari1327 是陆地棉半矮秆突变体。

Mapping of dwarfing QTL of Ari1327, a semi-dwarf mutant of upland cotton.

机构信息

State Key Laboratory of cotton Biology, Institute of Cotton Research of the Chinese Academy of Agricultural Sciences, Anyang, 455000, China.

Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, 450000, China.

出版信息

BMC Plant Biol. 2022 Jan 3;22(1):5. doi: 10.1186/s12870-021-03359-x.

BACKGROUND

Upland Cotton (Gossypium hirsutum L.) has few cotton varieties suitable for mechanical harvesting. The plant height of the cultivar is one of the key features that need to modify. Hence, this study was planned to locate the QTL for plant height in a Co γ treated upland cotton semi-dwarf mutant Ari1327.

RESULTS

Interestingly, bulk segregant analysis (BSA) and genotyping by sequencing (GBS) methods exhibited that candidate QTL was co-located in the region of 5.80-9.66 Mb at D01 chromosome in two F populations. Using three InDel markers to genotype a population of 1241 individuals confirmed that the offspring's phenotype is consistent with the genotype. Comparative analysis of RNA-seq between the mutant and wild variety exhibited that Gh_D01G0592 was identified as the source of dwarfness from 200 genes. In addition, it was also revealed that the appropriate use of partial separation markers in QTL mapping can escalate linkage information.

CONCLUSIONS

Overwhelmingly, the results will provide the basis to reveal the function of candidate genes and the utilization of excellent dwarf genetic resources in the future.

背景

陆地棉（Gossypium hirsutum L.）缺乏适合机械收获的棉花品种。株高是需要改良的关键特征之一。因此，本研究计划定位 Co γ 处理的陆地棉半矮突变体 Ari1327 中株高的 QTL。

结果

有趣的是，BSA 和 GBS 方法的群体分离分析表明，候选 QTL在两个 F2 群体的 D01 染色体的 5.80-9.66 Mb 区域共定位。使用三个 InDel 标记对 1241 个个体的群体进行基因分型，证实了后代的表型与基因型一致。突变体和野生型之间的 RNA-seq 比较分析表明，Gh_D01G0592 是从 200 个基因中鉴定出的矮化来源。此外，还揭示了在 QTL 作图中适当使用部分分离标记可以提高连锁信息。