Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, China.
Mol Biol Rep. 2011 Oct;38(7):4749-64. doi: 10.1007/s11033-010-0612-2. Epub 2010 Dec 5.
Barfin flounder (Verasper moseri) and spotted halibut (Verasper variegatus) are two economically important marine fish species for aquaculture in China, Korea and Japan. Construction of genetic linkage maps is an interesting issue for molecular marker-assisted selection (MAS) and for better understanding the genome structure. In the present study, we constructed genetic linkage maps for both fish species using AFLP and microsatellite markers based on an interspecific F(1) hybrid family (female V. moseri and male V. variegatus). The female genetic map comprised 98 markers (58 AFLP markers and 40 microsatellite markers), distributing in 27 linkage groups, and spanning 637 cM with an average resolution of 8.9 cM. Whereas the male genetic map consisted of 86 markers (48 AFLP and 38 microsatellite markers) in 24 linkage groups, covering a length of 625 cM with an average marker spacing of 10 cM. The expected genome length was 1,128 cM in female and 1,115 cM in male, and the estimated coverage of genome was 56% for both genetic maps. Moreover, five microsatellite markers were observed to be common to both genetic maps. This is the first time to report the genetic linkage maps of V. moseri and V. variegatus that could serve as the basis for genetic improvement and selective breeding, candidate genes cloning, and genome structure research.
圆斑星鲽（Verasper moseri）和斑点星鲽（Verasper variegatus）是中国、韩国和日本水产养殖中两种重要的经济鱼类。构建遗传连锁图谱对于分子标记辅助选择（MAS）以及更好地了解基因组结构非常有趣。本研究以圆斑星鲽♀和斑点星鲽♂杂交的 F1 代杂种家系为材料，利用 AFLP 和微卫星标记构建了这两种鱼的遗传连锁图谱。雌性遗传图谱包含 98 个标记（58 个 AFLP 标记和 40 个微卫星标记），分布在 27 个连锁群中，全长 637 cM，标记间平均距离为 8.9 cM。雄性遗传图谱包含 86 个标记（48 个 AFLP 标记和 38 个微卫星标记），分布在 24 个连锁群中，全长 625 cM，标记间平均距离为 10 cM。预期的雌性基因组大小为 1128 cM，雄性基因组大小为 1115 cM，两个图谱的基因组覆盖率分别为 56%。此外，在两个图谱中观察到 5 个微卫星标记共定位。这是首次报道圆斑星鲽和斑点星鲽的遗传连锁图谱，可为遗传改良和选育、候选基因克隆以及基因组结构研究提供基础。
