Ecron Acunova Ltd., Manipal, Karnataka 576104, India.
Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka 576104, India.
J Ethnopharmacol. 2018 Jan 10;210:118-124. doi: 10.1016/j.jep.2017.08.016. Epub 2017 Aug 19.
Tamarindus indica is an ingredient in the traditional aphrodisiac formulations in Africa and India. It is also a widely used food ingredient in other tropical countries.
The present study was aimed to evaluate the aphrodisiac potential and reproductive safety profile of aqueous extract of Tamarindus indica in male Wistar rats.
The aqueous extract was prepared by maceration of pulp followed by reduction of volume in rotavapor under heat followed by freeze drying. The prepared extract was characterized for contents of total phenol, flavonoid, and saponin. It was also subjected to phytoconstituent analysis using GCMS. Further, the extract was evaluated for acute toxicity study. The aphrodisiac and reproductive toxicity potential were evaluated in animals after grouping them in four with six animals each namely, normal control, standard (Sildenafil citrate, 4mg/kg p.o.) and extract of Tamarindus indica treated groups at two dose levels, 125 and 250mg/kg p.o. The study was conducted for 54 days with daily once dosing of extract and standard. Equal number of females was grouped without treatment for evaluation of parameters of sexual desire (mount frequency and intromission frequency) and parameters of sexual arousal (mount latency and intromission latency). These parameters were evaluated on day 14, 28, 42 and 54. Animals were sacrificed on day 54, testes were removed and studied for histopathological changes.
The extract showed 6.6mg gallic acid equivalent/g of total phenol, 2.3mg catechin equivalent/g of flavonoid and 11.6% saponin. Forty chemical constituents were identified by GCMS analysis. In acute toxicity study, the extract was found to be safe till 2000mg/kg p.o. Efficacy study showed significant (p<0.05) improvement in parameters of sexual desire (mount frequency and intromission frequency) and parameters of sexual arousal on all observed days except mount frequency for 125mg/kg on 42nd day and intromission frequency for both doses of tamarind compared to normal control. Improvements in these parameters were comparable to the standard drug. Histopathology study and sperm count suggested an increase in sperm production without any sign of toxicity in testis. Sperm motility significantly (p<0.05) increased in the treatment groups that received extract at 250mg/kg compared to normal control.
Aqueous extract of Tamarindus indica possessed aphrodisiac activity together with spermatogenic potential.
罗望子是非洲和印度传统壮阳配方中的一种成分。它也是其他热带国家广泛使用的食品成分。
本研究旨在评估罗望子水提物对雄性 Wistar 大鼠的壮阳潜力和生殖安全性。
通过在旋转蒸发器中加热浓缩果肉来制备水提物，然后冷冻干燥。对制备的提取物进行总酚、类黄酮和皂苷含量的特征分析。它还使用 GCMS 进行了植物成分分析。此外，还进行了急性毒性研究。将动物分为四组，每组 6 只，分别为正常对照组、标准组（枸橼酸西地那非，4mg/kg po）和罗望子提取物处理组，两个剂量水平，125 和 250mg/kg po。研究进行了 54 天，每天一次给药提取物和标准品。同样数量的雌性动物不接受治疗，用于评估性欲参数（交配频率和插入频率）和性唤起参数（交配潜伏期和插入潜伏期）。这些参数在第 14、28、42 和 54 天进行评估。动物在第 54 天被处死，取出睾丸进行组织病理学检查。
提取物显示 6.6mg 没食子酸当量/g 的总酚、2.3mg 儿茶素当量/g 的类黄酮和 11.6% 的皂苷。GCMS 分析鉴定了 40 种化学成分。在急性毒性研究中，提取物在 2000mg/kg po 时被发现是安全的。功效研究表明，与正常对照组相比，在所有观察到的日子里，性欲参数（交配频率和插入频率）和性唤起参数（交配潜伏期和插入潜伏期）都有显著（p<0.05）的改善，除了 125mg/kg 组在第 42 天的交配频率和罗望子的两种剂量的插入频率外。这些参数的改善与标准药物相当。组织病理学研究和精子计数表明，睾丸内精子生成增加，没有毒性迹象。与正常对照组相比，接受 250mg/kg 提取物治疗的治疗组的精子活力显著（p<0.05）增加。
罗望子水提物具有壮阳活性和生精潜力。
