Nayak Akshatha Ganesh, Kumar Nitesh, Shenoy Smita, Roche Maya
Department of Biochemistry, Melaka Manipal Medical College (Manipal Campus), Manipal Academy of Higher Education, Manipal-576104, Karnataka India.
Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal-576104, Karnataka India.
3 Biotech. 2020 Nov;10(11):476. doi: 10.1007/s13205-020-02462-4. Epub 2020 Oct 15.
The study investigates the ability of methanolic extract of (MAP) to supplement polyvalent anti-snake venom (ASV) in inhibiting neurotoxic enzyme acetylcholinesterase (AChE) and 'spreading factor' hyaluronidase from (N.N) venom. AChE and hyaluronidase activity were measured in 100 or 200 µg of crude venom, respectively, and designated as 'control'. In Test Group I, enzyme assays were performed immediately after the addition of ASV/MAP/ASV + MAP to the venom. Inhibition of AChE by ASV (100-367 µg) was 12-17%, and of hyaluronidase (22-660 µg) was 33-41%. Under the same conditions, MAP (100-400 µg) inhibited AChE and hyaluronidase to the extent of 17-33% and 17-52%, respectively. When ASV (220 µg) and MAP (100-200 µg) were added together, AChE and hyaluronidase were inhibited to a greater extent from 39-63 to 36-44%, than when either of them was used alone. In Test Group 2, the venom was incubated with ASV/MAP/ASV + MAP for 10-30 min at 37 °C prior to the assay which enhanced AChE inhibition by 6%, 82% and 18% respectively, when compared to Test Group I. Though there was no change in inhibition of hyaluronidase in the presence of ASV, MAP could further increase the extent of inhibition by 27% and ASV + MAP upto 4%. In Test Group III, venom and substrate were incubated for 90 min and hyaluronidase activity was measured after the addition of inhibitors. Here, ASV + MAP caused increased inhibition by 69% compared to ASV alone. The study confirms the ability of phytochemicals in MAP to contribute to a multipronged strategy by supplementing, thereby augmenting the efficacy of ASV.
该研究调查了[植物名称]甲醇提取物（MAP）在补充多价抗蛇毒血清（ASV）以抑制神经毒性酶乙酰胆碱酯酶（AChE）和[蛇名称]（N.N）毒液中的“扩散因子”透明质酸酶方面的能力。分别在100或200μg粗毒液中测量AChE和透明质酸酶活性，并将其指定为“对照”。在试验组I中，在向毒液中添加ASV/MAP/ASV + MAP后立即进行酶测定。ASV（100 - 367μg）对AChE的抑制率为12 - 17%，对透明质酸酶（22 - 660μg）的抑制率为33 - 41%。在相同条件下，MAP（100 - 400μg）对AChE和透明质酸酶的抑制率分别为17 - 33%和17 - 52%。当ASV（220μg）和MAP（100 - 200μg）一起添加时，与单独使用它们中的任何一种相比，AChE和透明质酸酶的抑制程度更大，从39 - 63%提高到36 - 44%。在试验组2中，在测定前将毒液与ASV/MAP/ASV + MAP在37℃下孵育10 - 30分钟，与试验组I相比，这分别使AChE抑制率提高了6%、82%和18%。尽管在ASV存在下透明质酸酶的抑制没有变化，但MAP可使抑制程度进一步提高27%，ASV + MAP可提高至4%。在试验组III中，将毒液和底物孵育90分钟，并在添加抑制剂后测量透明质酸酶活性。在这里，与单独使用ASV相比，ASV + MAP使抑制率提高了69%。该研究证实了MAP中的植物化学物质通过补充从而增强ASV的功效，有助于采取多管齐下策略的能力。
