Department of Experimental Medicine, Fuzhou General Hospital, 156 North Xi-er Huan Road, Fuzhou City, Fujian Province, 350025, China.
Department of General Surgery, Fuzhou General Hospital, 156 North Xi-er Huan Road, Fuzhou City, Fujian Province, 350025, China.
Sci Rep. 2017 Aug 22;7(1):9060. doi: 10.1038/s41598-017-09076-6.
Circular RNAs (circRNAs) are implicated in a variety of cancers. However, the roles of circRNAs in gastric cancer (GC) remain largely unknown. In the current study, circRNAs expression profiles were screened in GC, using 5 pairs of GC and matched non-GC tissues with circRNA chip. Preliminary results were verified with quantitative PCR (qRT-PCR). Briefly, total of 713 circRNAs were differentially expressed in GC tissues vs. non-GC tissues (fold change ≥ 2.0, p < 0.05): 191 were upregulated, whereas 522 were downregulated in GC tissues. qRT-PCR analysis of randomly selected 7 circRNAs from the 713 circRNAs in 50 paired of GC vs. non-GC control tissues confirmed the microarray data. Gene ontology (GO) and KEGG pathway analyses showed that many circRNAs are implicated in carcinogenesis. Among differentially expressed circRNAs, hsa_circ_0076304, hsa_circ_0035431, and hsa_circ_0076305 had the highest magnitude of change. These results provided a preliminary landscape of circRNAs expression profile in GC.
环状 RNA（circRNAs）与多种癌症有关。然而，circRNAs 在胃癌（GC）中的作用仍知之甚少。在本研究中，使用 circRNA 芯片对 5 对 GC 和匹配的非 GC 组织进行了 GC 中 circRNAs 表达谱的筛选。使用定量 PCR（qRT-PCR）对初步结果进行了验证。简而言之，与非 GC 组织相比，GC 组织中有 713 个 circRNAs 差异表达（倍数变化≥2.0，p<0.05）：191 个上调，522 个下调。对 50 对 GC 与非 GC 对照组织中的 713 个 circRNAs 中随机选择的 7 个 circRNAs 的 qRT-PCR 分析证实了微阵列数据。GO 和 KEGG 通路分析表明，许多 circRNAs 参与了致癌作用。在差异表达的 circRNAs 中，hsa_circ_0076304、hsa_circ_0035431 和 hsa_circ_0076305 的变化幅度最大。这些结果提供了 GC 中 circRNAs 表达谱的初步概况。
