Abdel-Aziz Swelum Ayman, M Saadeldin Islam, Ba-Awadh Hani, G Al-Mutary Mohsen, F Moumen Abdullah, N Alowaimer Abdullah, Abdalla Hany
Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia.
Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, 44519 Zagazig, Egypt.
Animals (Basel). 2019 Nov 19;9(11):999. doi: 10.3390/ani9110999.
This study compared the efficiency of commercial egg yolk-free (AndroMed, OPTIXcell) and egg yolk-supplemented (Triladyl, Steridyl) Tris-based extenders for semen cryopreservation in seven adult dromedary camels. The camel-specific extender SHOTOR was used as control. The collected semen samples were evaluated and diluted with SHOTOR, Triladyl, Steridyl, AndroMed, or OPTIXcell. The diluted semen was gradually cooled and equilibrated for two hours before liquid nitrogen freezing. Semen was evaluated prior to freezing and after freeze-thawing cycles for motility, kinetics, vitality, abnormality, plasma membrane integrity, and DNA fragmentation using computer-assisted sperm analysis. In pre-freezing evaluation, progressive sperm motility was higher in SHOTOR-diluted samples (21.54 ± 1.83) than in samples diluted with Steridyl, OPTIXcell, or AndroMed (15.76 ± 1.80, 17.43 ± 1.10, and 13.27 ± 1.07, respectively). Moreover, Triladyl and SHOTOR resulted in significantly ( < 0.05) better sperm vitality and DNA integrity than all other diluents, but Triladyl resulted in a significantly ( < 0.05) better plasma membrane integrity (87.77 ± 0.31) than SHOTOR (85.48 ± 0.58). In the post-thawing evaluation, Triladyl led to significantly ( < 0.05) higher sperm motility (38.63 ± 0.81%; < 0.05) when compared to SHOTOR, Steridyl or AndroMed (35.09 ± 1.341%, 34.4 ± 0.84%, and 31.99 ± 1.48%, respectively), with OPTIXcell being the least efficient (28.39 ± 0.86%). Progressive sperm motility was the highest when using Triladyl. Post-thawing curvilinear, straight line and average path sperm velocities were highest with Triladyl and lowest with AndroMed. Triladyl led to the highest linearity coefficient and straightness sperm coefficient, while SHOTOR to the highest DNA and plasma membrane integrity. OPTIXcell and AndroMed resulted in poor post-thawing sperm vitality, while Steridyl was less efficient than Triladyl. The highest rate of sperm abnormalities was recorded with OPTIXcell and the lowest with SHOTOR or Triladyl. In conclusion, SHOTOR, Triladyl, Steridyl, AndroMed, and OPTIXcell can all be used for camel semen cryopreservation; however, SHOTOR and Triladyl provided the best post-thawing sperm quality. Based on our findings, Triladyl is the best commercially available extender for dromedary camel semen cryopreservation to date.
本研究比较了七种成年单峰骆驼精液冷冻保存时，商业化无蛋黄（AndroMed、OPTIXcell）和添加蛋黄（Triladyl、Steridyl）的基于Tris的稀释液的效率。将骆驼专用稀释液SHOTOR用作对照。收集的精液样本经评估后，用SHOTOR、Triladyl、Steridyl、AndroMed或OPTIXcell进行稀释。稀释后的精液逐渐冷却并平衡两小时，然后进行液氮冷冻。在冷冻前和冻融循环后，使用计算机辅助精子分析对精液的活力、动力学、活力、异常情况、质膜完整性和DNA碎片化进行评估。在冷冻前评估中，用SHOTOR稀释的样本中渐进性精子活力（21.54±1.83）高于用Steridyl、OPTIXcell或AndroMed稀释的样本（分别为15.76±1.80、17.43±1.10和13.27±1.07）。此外，Triladyl和SHOTOR在精子活力和DNA完整性方面显著（<0.05）优于所有其他稀释剂，但Triladyl在质膜完整性方面显著（<0.05）优于SHOTOR（87.77±0.31）（85.48±0.58）。在解冻后评估中，与SHOTOR、Steridyl或AndroMed（分别为35.09±1.341%、34.4±0.84%和31.99±1.48%）相比，Triladyl导致精子活力显著（<0.05）更高（38.63±0.81%；<0.05），OPTIXcell效率最低（28.39±0.86%）。使用Triladyl时渐进性精子活力最高。解冻后的曲线、直线和平均路径精子速度在使用Triladyl时最高，在使用AndroMed时最低。Triladyl导致线性系数和直线性精子系数最高，而SHOTOR导致DNA和质膜完整性最高。OPTIXcell和AndroMed导致解冻后精子活力较差，而Steridyl的效率低于Triladyl。OPTIXcell记录的精子异常率最高，SHOTOR或Triladyl记录的最低。总之，SHOTOR、Triladyl、Steridyl、AndroMed和OPTIXcell均可用于骆驼精液冷冻保存；然而，SHOTOR和Triladyl提供了最佳的解冻后精子质量。根据我们的研究结果，Triladyl是迄今为止用于单峰骆驼精液冷冻保存的最佳商业化稀释剂。
