Wei Guangbing, Wu Yunhua, Gao Qi, Zhou Cancan, Wang Kai, Shen Cong, Wang Guanghui, Wang Kang, Sun Xuejun, Li Xuqi
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
Oxid Med Cell Longev. 2017;2017:1740317. doi: 10.1155/2017/1740317. Epub 2017 Aug 2.
Postoperative intra-abdominal adhesions are a major complication after abdominal surgery. Although various methods have been used to prevent and treat adhesions, the effects have not been satisfactory. Emodin, a naturally occurring anthraquinone derivative and an active ingredient in traditional Chinese herbs, exhibits a variety of pharmacological effects. In our study, we demonstrated the effect of emodin treatment on preventing postoperative adhesion formation.
A total of 48 rats were divided into six groups. Abdominal adhesions were created by abrasion of the cecum and its opposite abdominal wall. In the experimental groups, the rats were administered daily oral doses of emodin. On the seventh day after operation, the rats were euthanized, and blood and pathological specimens were collected. Abdominal adhesion formation was evaluated by necropsy, pathology, immunohistochemistry, Western blot, and enzyme-linked immunosorbent assay analyses.
Abdominal adhesions were markedly reduced by emodin treatment. Compared with the control group, collagen deposition was reduced and the peritoneal mesothelial completeness rate was higher in the emodin-treated groups. Emodin had anti-inflammatory effects, reduced oxidative stress, and promoted the movement of the intestinal tract ( < 0.05).
Emodin significantly reduced intra-abdominal adhesion formation in a rat model.
术后腹腔粘连是腹部手术后的主要并发症。尽管已采用多种方法预防和治疗粘连，但其效果并不理想。大黄素是一种天然存在的蒽醌衍生物，也是传统中药中的一种活性成分，具有多种药理作用。在我们的研究中，我们证明了大黄素治疗对预防术后粘连形成的作用。
总共48只大鼠被分为六组。通过盲肠及其相对的腹壁擦伤来造成腹腔粘连。在实验组中，大鼠每日口服大黄素。术后第七天，对大鼠实施安乐死，并采集血液和病理标本。通过尸检、病理学、免疫组织化学、蛋白质印迹法和酶联免疫吸附测定分析来评估腹腔粘连形成情况。
大黄素治疗显著减少了腹腔粘连。与对照组相比，大黄素治疗组的胶原沉积减少，腹膜间皮完整性率更高。大黄素有抗炎作用，降低氧化应激，并促进肠道蠕动（P<0.05）。
大黄素在大鼠模型中显著减少了腹腔粘连形成。
