Giusto Gessica, Vercelli Cristina, Iussich Selina, Audisio Andrea, Morello Emanuela, Odore Rosangela, Gandini Marco
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini, n. 2, Grugliasco, Turin, 10095, Italy.
BMC Vet Res. 2017 Feb 17;13(1):55. doi: 10.1186/s12917-017-0965-z.
Adhesions are a common postoperative surgical complication. Liquid honey has been used intraperitoneally to reduce the incidence of these adhesions. However, solid barriers are considered more effective than liquids in decreasing postoperative intra-abdominal adhesion formation; therefore, a new pectin-honey hydrogel (PHH) was produced and its effectiveness was evaluated in a rat cecal abrasion model. Standardized cecal/peritoneal abrasion was performed through laparotomy in 48 adult Sprague-Dawley rats to induce peritoneal adhesion formation. Rats were randomly assigned to a control (C) and treatment (T) group. In group T, PHHs were placed between the injured peritoneum and cecum. Animals were euthanized on day 15 after surgery. Adhesions were evaluated macroscopically and adhesion scores were recorded and compared between the two groups. Inflammation, fibrosis, and neovascularization were histologically graded and compared between the groups.
In group C, 17 of 24 (70.8%) animals developed adhesions between the cecum and peritoneum, while in group T only 5 of 24 (20.8%) did (p = 0.0012). In group C, one rat had an adhesion score of 3, sixteen had scores of 2, and seven rats had scores of 0. In group T, four rats had adhesion scores of 2, one rat had an adhesion score of 1 and nineteen have score 0 (p = 0.0003). Significantly lower grades of inflammation, fibrosis, and neovascularization were seen in group T (p = 0.006, p = 0.001, p = 0.002, respectively).
PHH is a novel absorbable barrier that is effective in preventing intra-abdominal adhesions in a cecal abrasion model in rats.
粘连是一种常见的术后手术并发症。液态蜂蜜已被用于腹腔内以降低这些粘连的发生率。然而，在减少术后腹腔内粘连形成方面，固体屏障被认为比液体更有效；因此，制备了一种新型果胶-蜂蜜水凝胶（PHH），并在大鼠盲肠擦伤模型中评估了其有效性。通过剖腹术对48只成年Sprague-Dawley大鼠进行标准化的盲肠/腹膜擦伤，以诱导腹膜粘连形成。大鼠被随机分为对照组（C）和治疗组（T）。在T组中，将PHH置于受损的腹膜和盲肠之间。在手术后第15天对动物实施安乐死。宏观评估粘连情况，并记录粘连评分，比较两组之间的差异。对两组之间的炎症、纤维化和新生血管形成进行组织学分级和比较。
在C组中，24只动物中有17只（70.8%）在盲肠和腹膜之间形成了粘连，而在T组中，24只动物中只有5只（20.8%）形成了粘连（p = 0.0012）。在C组中，一只大鼠的粘连评分为3分，16只大鼠评分为2分，7只大鼠评分为0分。在T组中，4只大鼠的粘连评分为2分，1只大鼠评分为1分，19只大鼠评分为0分（p = 0.0003）。T组的炎症、纤维化和新生血管形成程度明显较低（分别为p = 0.006、p = 0.001、p = 0.002）。
PHH是一种新型可吸收屏障，在大鼠盲肠擦伤模型中可有效预防腹腔内粘连。
