Department of Internal Medicine, Pulmonology and Allergology, Medical University of Warsaw, Warsaw, Poland.
Laboratory of Flow Cytometry and Hematology, Department of Internal Medicine and Hematology, Military Medical Institute, Warsaw, Poland.
Biomed Res Int. 2017;2017:5185034. doi: 10.1155/2017/5185034. Epub 2017 Aug 2.
A balance between tumor invasion and immune defence system is widely investigated. . The aim of this study was to evaluate lymphocyte phenotype in lymph nodes (LNs) of patients with lung cancer in relation to the presence of metastases. . We investigated 364 LNs resected by transcervical extended mediastinal lymphadenectomy (TEMLA) of 49 patients with squamous cell carcinoma (SCC) or adenocarcinoma (AD) with (A) and without metastases (B). Expression of CD4, CD8, CD25, CTLA-4, and Foxp3 was assessed by immunohistochemical staining. . We observed a strong nuclear staining for Foxp3 in lymphocytes and cancer cells and strong membranous/cytoplasmatic reaction for CD4 and CD8, but low for CD25 and CTLA-4. There were significantly higher proportions of CD8+ cells in AD (B) versus AD (A) LNs (80% versus 52.5%, < 0.05). The Foxp3/CD8 ratio was higher in AD (A) versus AD (B) LNs (0.4 versus 0.25, < 0.05). No significant differences in the cell markers expression in SCC LNs were found. . Significant differences in lymphocyte phenotype in AD may indicate an exceptional biology of this type of lung cancer. TEMLA resected LNs may serve as valuable samples for evaluation of immune status in lung cancer patients.
肿瘤侵袭与免疫系统之间的平衡受到广泛研究。本研究旨在评估肺癌患者淋巴结中的淋巴细胞表型与转移的关系。我们研究了 49 例经颈前路纵隔扩大淋巴结清扫术（TEMLA）切除的 364 个淋巴结，这些患者患有鳞状细胞癌（SCC）或腺癌（AD），并分为有转移（A）和无转移（B）两组。通过免疫组织化学染色评估 CD4、CD8、CD25、CTLA-4 和 Foxp3 的表达。我们观察到淋巴细胞和癌细胞中 Foxp3 呈强核染色，CD4 和 CD8 呈强膜/细胞质反应，但 CD25 和 CTLA-4 呈低反应。AD（B）淋巴结中的 CD8+细胞比例明显高于 AD（A）淋巴结（80%比 52.5%，<0.05）。AD（A）淋巴结中的 Foxp3/CD8 比值高于 AD（B）淋巴结（0.4 比 0.25，<0.05）。在 SCC 淋巴结中，细胞标志物的表达无显著差异。AD 中淋巴细胞表型的显著差异可能表明这种类型的肺癌具有特殊的生物学特性。TEMLA 切除的淋巴结可作为评估肺癌患者免疫状态的有价值样本。
