Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Institute Building 3, Phayathai Road, Wangmai, Pathumwan, Bangkok, 10330, Thailand.
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
Bioprocess Biosyst Eng. 2017 Dec;40(12):1787-1799. doi: 10.1007/s00449-017-1833-8. Epub 2017 Aug 22.
Bacillus sp. BC-001 was first reported as a potent thermotolerant and homofermentative strain for an industrial-scale L-lactate production. In a flask culture, this isolate fermented both glucose and sucrose to lactate with high yield (0.96 and 0.87 g/g) and productivity (2.8 and 2.6 g/L h), respectively. The higher lactate production performance was obtained in the simultaneous saccharification and fermentation of liquefied starch (150.1 g/L final titer, 0.98 g/g yield, 3.2 g/L h productivity) and the fed-batch glucose fermentation (139.9 g/L final titer, 0.96 g/g yield, 2.9 g/L h productivity). Significant increase in lactate productivity (5.5-6.1 g/L h) was obtained from the high/heavy-inoculum seed in the stirred tank fermentor. Both calcium bases and monovalent bases were successfully employed for pH control during lactate fermentation by this isolate resulting in a versatile and simple operation. By the two-phase fermentation using the high/heavy-inoculum seed of BC-001, the fermentation reproducibility was acquired toward the pilot-scale fermentors.
芽孢杆菌 BC-001 最初被报道为一种具有耐热和同型发酵能力的菌株，可用于工业规模的 L-乳酸生产。在摇瓶培养中，该分离株分别以高得率（0.96 和 0.87 g/g）和高生产率（2.8 和 2.6 g/L/h）发酵葡萄糖和蔗糖生产乳酸。在液化淀粉的同步糖化和发酵（最终浓度为 150.1 g/L，产率为 0.98 g/g，生产率为 3.2 g/L/h）和分批补料葡萄糖发酵（最终浓度为 139.9 g/L，产率为 0.96 g/g，生产率为 2.9 g/L/h）中获得了更高的乳酸生产性能。在搅拌罐发酵器中，通过高/重接种种子，乳酸生产率显著提高（5.5-6.1 g/L/h）。该分离株在乳酸发酵过程中成功地使用钙盐和单价盐来控制 pH 值，从而实现了灵活简单的操作。通过使用高/重接种种子的两相发酵，在中试规模发酵罐中获得了发酵重现性。
