Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology , Qingdao 266042, China.
College of Chemistry and Chemical Engineering, Taishan University , Taian 271021, China.
ACS Appl Mater Interfaces. 2017 Sep 13;9(36):31153-31160. doi: 10.1021/acsami.7b09529. Epub 2017 Aug 30.
Detection of disease biomarkers within complex biological media is a substantial outstanding challenge because of severe biofouling and nonspecific adsorptions. Herein, a reliable strategy for sensitive and low-fouling detection of a biomarker, adenosine triphosphate (ATP) in biological samples was developed through the formation of a mixed self-assembled sensing interface, which was constructed by simultaneously self-assembling polyethylene glycol (PEG) and ATP aptamer onto the self-polymerized polydopamine-modified electrode surface. The developed aptasensor exhibited high selectivity and sensitivity toward the detection of ATP, and the linear range was 0.1-1000 pM, with a detection limit down to 0.1 pM. Moreover, owing to the presence of PEG within the sensing interface, the aptasensor was capable of sensing ATP in complex biological media such as human plasma with significantly reduced nonspecific adsorption effect. Assaying ATP in real biological samples including breast cancer cell lysates further proved the feasibility of this biosensor for practical application.
在复杂的生物介质中检测疾病生物标志物是一个重大的挑战，因为会存在严重的生物污垢和非特异性吸附。在此，通过形成混合自组装传感界面，开发了一种用于生物样品中生物标志物三磷酸腺苷 (ATP) 的灵敏、低污染检测的可靠策略，该界面是通过将聚乙二醇 (PEG) 和 ATP 适体同时自组装到自聚合聚多巴胺修饰电极表面上构建的。所开发的适体传感器对 ATP 的检测表现出高选择性和灵敏度，线性范围为 0.1-1000 pM，检测限低至 0.1 pM。此外，由于传感界面内存在 PEG，该适体传感器能够在复杂的生物介质（如人血浆）中检测 ATP，同时显著降低非特异性吸附效应。在包括乳腺癌细胞裂解物在内的实际生物样品中测定 ATP 进一步证明了该生物传感器用于实际应用的可行性。
