Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology , Qingdao 266042, China.
ACS Appl Mater Interfaces. 2017 Jan 25;9(3):2914-2923. doi: 10.1021/acsami.6b11682. Epub 2017 Jan 10.
Biofouling arising from nonspecific adsorption is a substantial outstanding challenge in diagnostics and disease monitoring, and antifouling sensing interfaces capable of reducing the nonspecific adsorption of proteins from biological complex samples are highly desirable. We present herein the preparation of novel composite nanofibers through the grafting of polyethylene glycol (PEG) polymer onto polyaniline (PANI) nanofibers and their application in the development of antifouling electrochemical biosensors. The PEGylated PANI (PANI/PEG) nanofibers possessed large surface area and remained conductive and at the same time demonstrated excellent antifouling performances in single protein solutions as well as complex human serum samples. Sensitive and low fouling electrochemical biosensors for the breast cancer susceptibility gene (BRCA1) can be easily fabricated through the attachment of DNA probes to the PANI/PEG nanofibers. The biosensor showed a very high sensitivity to target BRCA1 with a linear range from 0.01 pM to 1 nM and was also efficient enough to detect DNA mismatches with satisfactory selectivity. Moreover, the DNA biosensor based on the PEGylated PANI nanofibers supported the quantification of BRCA1 in complex human serum, indicating great potential of this novel biomaterial for application in biosensors and bioelectronics.
生物污垢源于非特异性吸附，这是诊断和疾病监测中一个重大的难题，因此需要开发具有抗污染性能的传感界面，以减少生物复杂样品中蛋白质的非特异性吸附。本文通过将聚乙二醇（PEG）聚合物接枝到聚苯胺（PANI）纳米纤维上，制备了新型复合纳米纤维，并将其应用于开发抗污染电化学生物传感器。PEG 化的聚苯胺（PANI/PEG）纳米纤维具有较大的比表面积，保持了导电性，同时在单蛋白溶液和复杂的人血清样品中表现出优异的抗污染性能。通过将 DNA 探针固定在 PANI/PEG 纳米纤维上，很容易制备出用于检测乳腺癌易感性基因（BRCA1）的灵敏且抗污染的电化学生物传感器。该生物传感器对靶标 BRCA1 具有很高的灵敏度，线性范围从 0.01 pM 到 1 nM，并且对 DNA 错配的检测也具有足够的选择性。此外，基于 PEG 化 PANI 纳米纤维的 DNA 生物传感器可以对复杂的人血清中的 BRCA1 进行定量分析，这表明这种新型生物材料在生物传感器和生物电子学领域具有很大的应用潜力。
