Lee Jung Nam, Wang Yong, Xu Ya Ou, Li Yu Can, Tian Fang, Jiang Ming Feng
College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China.
Sichuan Key Laboratory of Conservation and Utilization of Animal Genetic Resources in Tibetan Plateau,Southwest University for Nationalities,Chengdu 610041,China.
J Dairy Res. 2017 Aug;84(3):283-288. doi: 10.1017/S0022029917000413.
This research communication describes the profile of gene expression related to the synthesis of yak milk as determined via quantitative reverse transcription polymerase chain reaction (RT-qPCR). Significant up-regulation during lactation were observed in genes related to fatty acid (FA) uptake from blood (LPL, CD36), intracellular FA transport (FABP3), intracellular FA activation of long- and short-chain FAs (ACSS1, ACSS2, ACSL1), de novo synthesis (ACACA), desaturation (SCD), triacyglycerol (TAG) synthesis (AGPAT6, GPAM, LPIN1), lipid droplet formation (PLIN2, BTN1A1, XDH), ketone body utilisation (BDH1, OXCT1), and transcription regulation (THRSP, PPARGC1A). In particular, intracellular de novo FA synthesis (ACSS2, ACACA, and FABP3) and TAG synthesis (GPAM, AGPAT6, and LPIN1), whose regulation might be orchestrated as part of the gene network under the control of SERBF1 in the milk fat synthesis process, were more activated compared to levels in dairy cows. However, the genes involved in lipid droplet formation (PLIN2, XDH, and BTN1A1) were expressed at lower levels compared to those in dairy cows, where these genes are mainly controlled by the PPARG regulator.
本研究通讯描述了通过定量逆转录聚合酶链反应（RT-qPCR）确定的与牦牛乳合成相关的基因表达概况。在泌乳期间，观察到与从血液中摄取脂肪酸（FA）（LPL、CD36）、细胞内FA转运（FABP3）、长链和短链FA的细胞内FA激活（ACSS1、ACSS2、ACSL1）、从头合成（ACACA）、去饱和（SCD）、三酰甘油（TAG）合成（AGPAT6、GPAM、LPIN1）、脂滴形成（PLIN2、BTN1A1、XDH）、酮体利用（BDH1、OXCT1）以及转录调控（THRSP、PPARGC1A）相关的基因显著上调。特别是，细胞内从头FA合成（ACSS2、ACACA和FABP3）以及TAG合成（GPAM、AGPAT6和LPIN1），其调控可能作为乳脂肪合成过程中受SERBF1控制的基因网络的一部分进行协调，与奶牛相比，其激活程度更高。然而，与奶牛相比，参与脂滴形成的基因（PLIN2、XDH和BTN1A1）表达水平较低，在奶牛中这些基因主要受PPARG调节因子控制。
