Perteghella Sara, Martella Elisa, de Girolamo Laura, Perucca Orfei Carlotta, Pierini Michela, Fumagalli Valentina, Pintacuda Domenica Valeria, Chlapanidas Theodora, Viganò Marco, Faragò Silvio, Torre Maria Luisa, Lucarelli Enrico
Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Via Zamboni 33, 40126 Bologna, Italy.
Int J Mol Sci. 2017 Aug 23;18(9):1829. doi: 10.3390/ijms18091829.
The aim of this study was to exploit silk fibroin's properties to develop innovative composite microcarriers for mesenchymal stem cell (MSCs) adhesion and proliferation. Alginate microcarriers were prepared, added to silk fibroin solution, and then treated with ethanol to induce silk conformational transition. Microcarriers were characterized for size distribution, coating stability and homogeneity. Finally, in vitro cytocompatibility and suitability as delivery systems for MSCs were investigated. Results indicated that our manufacturing process is consistent and reproducible: silk/alginate microcarriers were stable, with spherical geometry, about 400 μm in average diameter, and fibroin homogeneously coated the surface. MSCs were able to adhere rapidly onto the microcarrier surface and to cover the surface of the microcarrier within three days of culture; moreover, on this innovative 3D culture system, stem cells preserved their metabolic activity and their multi-lineage differentiation potential. In conclusion, silk/alginate microcarriers represent a suitable support for MSCs culture and expansion. Since it is able to preserve MSCs multipotency, the developed 3D system can be intended for cell delivery, for advanced therapy and regenerative medicine applications.
本研究的目的是利用丝素蛋白的特性，开发用于间充质干细胞（MSC）黏附与增殖的新型复合微载体。制备藻酸盐微载体，将其加入丝素蛋白溶液中，然后用乙醇处理以诱导丝素蛋白构象转变。对微载体的尺寸分布、包被稳定性和均匀性进行了表征。最后，研究了其体外细胞相容性以及作为MSC递送系统的适用性。结果表明，我们的制造工艺具有一致性和可重复性：丝素蛋白/藻酸盐微载体稳定，呈球形几何形状，平均直径约400μm，丝素蛋白均匀地包被在表面。MSC能够迅速黏附到微载体表面，并在培养三天内覆盖微载体表面；此外，在这种新型的三维培养系统上，干细胞保留了它们的代谢活性和多向分化潜能。总之，丝素蛋白/藻酸盐微载体是MSC培养和扩增的合适载体。由于它能够保留MSC的多能性，所开发的三维系统可用于细胞递送、先进治疗和再生医学应用。
Zotero 插件
