Bardyn M, Maye S, Lesch A, Delobel J, Tissot J-D, Cortés-Salazar F, Tacchini P, Lion N, Girault H H, Prudent M
Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, Epalinges, Switzerland.
Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, EPFL Valais Wallis, Sion, Switzerland.
Vox Sang. 2017 Oct;112(7):638-647. doi: 10.1111/vox.12563. Epub 2017 Aug 18.
Red blood cells (RBCs) suffer from lesions during cold storage, depending in part on their ability to counterbalance oxidative stress by activating their antioxidant defence. The aim of this study was to monitor the antioxidant power (AOP) in erythrocyte concentrates (ECs) during cold storage.
Six ECs were prepared in saline-adenine-glucose-mannitol (SAGM) additive solution and followed during 43 days. The AOP was quantified electrochemically using disposable electrode strips and compared with results obtained from a colorimetric assay. Haematological data, data on haemolysis and the extracellular concentration of uric acid were also recorded. Additionally, a kinetic model was developed to extract quantitative kinetic data on the AOP behaviour.
The AOP of total ECs and their extracellular samples attained a maximum after 1 week of storage prior to decaying and reaching a plateau, as shown by the electrochemical measurements. The observed trend was confirmed with a colorimetric assay. Uric acid had a major contribution to the extracellular AOP. Interestingly, the AOP and uric acid levels were linked to the sex of the donors.
The marked increase in AOP during the first week of storage suggests that RBCs are impacted early by the modification of their environment. The AOP behaviour reflects the changes in metabolism activity following the adjustment of the extracellular uric acid level. Knowing the origin, interdonor variability and the effects of the AOP on the RBCs could be beneficial for the storage quality, which will have to be further studied.
红细胞在冷藏过程中会受到损伤，这部分取决于它们通过激活抗氧化防御来平衡氧化应激的能力。本研究的目的是监测冷藏期间红细胞浓缩液（ECs）的抗氧化能力（AOP）。
在生理盐水 - 腺嘌呤 - 葡萄糖 - 甘露醇（SAGM）添加剂溶液中制备6份ECs，并在43天内进行跟踪监测。使用一次性电极条通过电化学方法对AOP进行定量，并与比色法获得的结果进行比较。还记录了血液学数据、溶血数据和细胞外尿酸浓度。此外，建立了一个动力学模型来提取关于AOP行为的定量动力学数据。
电化学测量结果显示，总ECs及其细胞外样品的AOP在储存1周后达到最大值，随后下降并趋于平稳。比色法证实了观察到的趋势。尿酸对细胞外AOP有主要贡献。有趣的是，AOP和尿酸水平与供体的性别有关。
储存第一周AOP的显著增加表明红细胞早期受到其环境变化的影响。AOP行为反映了细胞外尿酸水平调整后代谢活性的变化。了解AOP的来源、供体间变异性及其对红细胞的影响可能有助于储存质量，这还有待进一步研究。
