Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, 1066 Epalinges, Switzerland.
Faculté de Biologie et de Médecine, University of Lausanne, 1011 Lausanne, Switzerland.
Cells. 2023 May 23;12(11):1454. doi: 10.3390/cells12111454.
The hypothesis of the potential impact of the sex of red blood cell (RBC) concentrate (RCC) donors, as well as the sex of the recipients, on the clinical outcome, is still under evaluation. Here, we have evaluated the sex impact on RBC properties using in vitro transfusion models. Using a "flask model", RBCs from RCCs (representing the donor)-at different storage lengths-were incubated in a sex-matched and sex-mismatched manner with fresh frozen plasma pools (representing the recipient) at 37 °C, with 5% of CO up to 48 h. Standard blood parameters, hemolysis, intracellular ATP, extracellular glucose and lactate were quantified during incubation. Additionally, a "plate model", coupling hemolysis analysis and morphological study, was carried out in similar conditions in 96-well plates. In both models, RBCs from both sexes hemolyzed significantly less in female-derived plasma. No metabolic or morphological differences were observed between sex-matched and -mismatched conditions, even though ATP was higher in female-derived RBCs during incubations. Female plasma reduced hemolysis of female- as well as male-derived RBCs, which may be related to a sex-dependent plasma composition and/or sex-related intrinsic RBC properties.
关于红细胞（RBC）浓缩物（RCC）供体的性别以及受者的性别对临床结果的潜在影响的假设，仍在评估中。在这里，我们使用体外输血模型评估了性别的影响对 RBC 特性的影响。使用“瓶模型”，在 37°C 下，将来自 RCC（代表供体）的 RBC 在不同储存时间下以性别匹配和性别不匹配的方式与新鲜冷冻血浆池（代表受者）孵育，直到 48 小时，同时孵育过程中定量了标准血液参数、溶血、细胞内 ATP、细胞外葡萄糖和乳酸。此外，在类似条件下，还在 96 孔板中进行了“板模型”，结合溶血分析和形态研究。在两种模型中，来自两性的 RBC 在女性来源的血浆中溶血明显较少。即使在孵育过程中，来自女性的 RBC 中的 ATP 较高，在性别匹配和不匹配条件之间也未观察到代谢或形态差异。女性血浆减少了女性和男性来源的 RBC 的溶血，这可能与性别相关的血浆组成和/或与性别相关的内在 RBC 特性有关。
