Zhang Jing, Wang Tenghua, Ren Zhiyao, Gong Lu, Huang Juan, Bai Junqi, Qiu Xiaohui, Xu Wen
Laboratory of Chinese Materia Medica Preparation, The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China.
Guangdong Provincial Academy of Chinese medical sciences, Guangzhou, China.
Biomed Chromatogr. 2018 Feb;32(2). doi: 10.1002/bmc.4067. Epub 2017 Sep 14.
The root of Polygonum multiflorum (PM) is an important Chinese herbal medicine for treatment of various diseases. Extensive pharmacological studies have been conducted and demonstrated that it shows a wide range of bioactivities. Meanwhile, a considerable number of hepatotoxicity cases owing to oral administration of PM have been reported and have attracted great attention. However, the limited knowledge regarding the metabolism of PM restricts the deeper studies on its pharmacological/toxicological mechanism and therapeutic material basis. The present study aimed to develop a high-performance liquid chromatography coupled with a linear ion trap-Orbitrap hybrid mass spectrometry method for separation and identification of metabolites in rat urine and plasma after oral administration of PM. Based on the proposed strategy, metabolism profiles of PM in rats were proposed for the first time and 43 metabolites were characterized or tentatively identified. Phase II metabolism, such as glucuronidation and sulfation, are the principal pathways of the main components. These findings will be beneficial to further understanding of the pharmacological mechanism and pharmacodynamic material basis of PM.
何首乌的根是一种用于治疗多种疾病的重要中药材。已开展了广泛的药理学研究，结果表明其具有多种生物活性。与此同时，已有相当数量因口服何首乌而导致肝毒性的病例报道，并引起了广泛关注。然而，关于何首乌代谢的知识有限，这限制了对其药理/毒理机制及治疗物质基础的深入研究。本研究旨在建立一种高效液相色谱-线性离子阱-轨道阱混合质谱联用方法，用于口服何首乌后大鼠尿液和血浆中代谢产物的分离与鉴定。基于所提出的策略，首次提出了何首乌在大鼠体内的代谢谱，并鉴定或初步鉴定了43种代谢产物。Ⅱ相代谢，如葡萄糖醛酸化和硫酸化，是主要成分的主要代谢途径。这些发现将有助于进一步了解何首乌的药理机制和药效物质基础。
