Lab of Chinese Materia Medica Preparation, The Second School of Clinic Medicine, Guangzhou University of Chinese Medicine, Guangdong Province Institute of TCM, Guangzhou, China.
J Chromatogr A. 2013 May 31;1292:121-31. doi: 10.1016/j.chroma.2012.11.051. Epub 2012 Nov 26.
A simple and effective method was developed for characterization of phenolic constituents in the roots of Polygonum multiflorum by combination of ultra-high-pressure liquid chromatography with linear ion trap-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap). Stilbenes, anthraquinones, tannins and naphthalenes were differentiated by diagnostic fragment ions with accurate mass measurements and characteristic fragmentation pathways. Based on the proposed strategy, fifty-nine constituents were characterized or tentatively identified, of which twenty-two constituents were the first to be reported in P. multiflorum and twelve compounds were characterized as potential new compounds. The identification and structure elucidation of these chemicals provided essential data for further phytochemical studies and quality control of P. multiflorum. The results also demonstrated that our novel method can be extended to screen and characterize other phenolic constituents and their metabolites in botanical extracts.
建立了一种超高效液相色谱-线性离子阱-Orbitrap 串联质谱(UHPLC-LTQ-Orbitrap)联用技术，用于分析何首乌根中酚类成分的特征。通过精确质量测量和特征裂解途径的诊断碎片离子，区分了芪类、蒽醌类、鞣质和萘类。基于所提出的策略，鉴定或初步鉴定了 59 种成分，其中 22 种成分是首次在何首乌中报道的，12 种化合物被鉴定为潜在的新化合物。这些化合物的鉴定和结构阐明为进一步的植物化学研究和何首乌的质量控制提供了必要的数据。研究结果还表明，我们的新方法可以扩展到筛选和鉴定植物提取物中的其他酚类成分及其代谢产物。
