Privalsky M L
J Virol. 1987 Jun;61(6):1938-48. doi: 10.1128/JVI.61.6.1938-1948.1987.
A novel gene was created that linked complementary portions of two different tyrosine kinase oncogenes: v-erB and v-src. The v-erbB/src chimera encoded a glycoprotein exhibiting the subcellular distribution of the v-erbB protein but containing the kinase catalytic domain of the v-src parent. Fibroblasts expressing the v-erbB/src gene product became transformed to an oncogenic state and closely resembled cells expressing the v-erbB parent oncogene. Our results indicated that v-erbB sequences can be functionally replaced by sequences derived from a different oncogene, v-src, and that important determinants of the transformed phenotype appear to be encoded in oncogene sequences distinct from those defining the kinase catalytic domain itself.
构建了一个新基因，它连接了两种不同酪氨酸激酶癌基因的互补部分：v-erB和v-src。v-erbB/src嵌合体编码一种糖蛋白，该糖蛋白呈现v-erB蛋白的亚细胞分布，但含有v-src亲本的激酶催化结构域。表达v-erbB/src基因产物的成纤维细胞转变为致癌状态，并且与表达v-erB亲本癌基因的细胞极为相似。我们的结果表明，v-erB序列可被源自不同癌基因v-src的序列功能性替代，并且转化表型的重要决定因素似乎由不同于定义激酶催化结构域本身的癌基因序列编码。
