Goncalves Priscila, Jones David B, Thompson Emma L, Parker Laura M, Ross Pauline M, Raftos David A
Department of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
Sydney Institute of Marine Science, Sydney, NSW, Australia.
Mol Ecol. 2017 Nov;26(21):5974-5988. doi: 10.1111/mec.14333. Epub 2017 Sep 29.
Some populations of marine organisms appear to have inherent tolerance or the capacity for acclimation to stressful environmental conditions, including those associated with climate change. Sydney rock oysters from the B2 breeding line exhibit resilience to ocean acidification (OA) at the physiological level. To understand the molecular basis of this physiological resilience, we analysed the gill transcriptome of B2 oysters that had been exposed to near-future projected ocean pH over two consecutive generations. Our results suggest that the distinctive performance of B2 oysters in the face of OA is mediated by the selective expression of genes involved in multiple cellular processes. Subsequent high-throughput qPCR revealed that some of these transcriptional changes are exclusive to B2 oysters and so may be associated with their resilience to OA. The intracellular processes mediated by the differentially abundant genes primarily involve control of the cell cycle and maintenance of cellular homeostasis. These changes may enable B2 oysters to prevent apoptosis resulting from oxidative damage or to alleviate the effects of apoptosis through regulation of the cell cycle. Comparative analysis of the OA conditioning effects across sequential generations supported the contention that B2 and wild-type oysters have different trajectories of changing gene expression and responding to OA. Our findings reveal the broad set of molecular processes underlying transgenerational conditioning and potential resilience to OA in a marine calcifier. Identifying the mechanisms of stress resilience can uncover the intracellular basis for these organisms to survive and thrive in a rapidly changing ocean.
一些海洋生物种群似乎具有内在的耐受性或适应压力环境条件的能力，包括与气候变化相关的条件。来自B2育种系的悉尼岩牡蛎在生理水平上表现出对海洋酸化（OA）的恢复力。为了了解这种生理恢复力的分子基础，我们分析了连续两代暴露于未来预计海洋pH值的B2牡蛎的鳃转录组。我们的结果表明，B2牡蛎在面对OA时的独特表现是由参与多个细胞过程的基因的选择性表达介导的。随后的高通量qPCR显示，其中一些转录变化是B2牡蛎特有的，因此可能与其对OA的恢复力有关。差异丰富基因介导的细胞内过程主要涉及细胞周期的控制和细胞内稳态的维持。这些变化可能使B2牡蛎能够预防氧化损伤导致的细胞凋亡，或通过调节细胞周期减轻细胞凋亡的影响。对连续几代OA条件作用的比较分析支持了这样的观点，即B2牡蛎和野生型牡蛎在基因表达变化和对OA反应方面有不同的轨迹。我们的研究结果揭示了海洋钙化生物跨代条件作用和对OA潜在恢复力的广泛分子过程。确定压力恢复力的机制可以揭示这些生物在快速变化的海洋中生存和繁衍的细胞内基础。
