Kammerlander Barbara, Tartarotti Barbara, Sonntag Bettina
Lake and Glacier Research Group, Institute of Ecology, University of Innsbruck, Technikerstrasse 25, Innsbruck, 6020, Austria.
Ciliate Ecology and Taxonomy Group, Research Department for Limnology, Mondsee, University of Innsbruck, Mondseestrasse 9, Mondsee, 5310, Austria.
J Eukaryot Microbiol. 2018 Mar;65(2):250-254. doi: 10.1111/jeu.12463. Epub 2017 Sep 8.
Paramecium populations from a clear and a glacier-fed turbid alpine lake were exposed to solar simulated ultraviolet (UVR) and photosynthetically active radiation (PAR) at 8 and 15 °C. The ciliates were tested for DNA damage (comet assay), behavioral changes, and mortality after UVR + PAR exposure. High DNA damage levels (~58% tail DNA) and abnormal swimming behavior were observed, although no significant changes in cell numbers were found irrespective of the lake origin (clear, turbid), and temperatures. We conclude that environmental stressors such as UVR and their effects may influence the adaptation of ciliates living in alpine lakes.
将来自一个清澈的和一个由冰川融水补给的浑浊高山湖泊的草履虫种群，分别置于8摄氏度和15摄氏度的条件下，使其暴露于模拟太阳紫外线（UVR）和光合有效辐射（PAR）中。对这些纤毛虫进行了紫外线辐射+光合有效辐射暴露后的DNA损伤（彗星试验）、行为变化和死亡率测试。尽管无论湖泊来源（清澈、浑浊）和温度如何，细胞数量均未发现显著变化，但仍观察到了高水平的DNA损伤（约58%的尾DNA）和异常的游泳行为。我们得出结论，紫外线辐射等环境压力源及其影响可能会影响生活在高山湖泊中的纤毛虫的适应性。
