Maeda Seishi, Fujihira Mayumi, Minato Yusuke, Kuwahara-Otani Sachi, Tanaka Koichi, Hayakawa Tetsu, Yagi Hideshi
Department of Anatomy and Cell Biology, Hyogo College of Medicine, 1-1 Mukogawa, Nishinomiya, Hyogo 663-8501, Japan.
Laboratory of Tumor Immunology and Cell Therapy, Hyogo College of Medicine, 1-1 Mukogawa, Nishinomiya, Hyogo 663-8501, Japan.
Anat Rec (Hoboken). 2017 Dec;300(12):2263-2272. doi: 10.1002/ar.23680. Epub 2017 Aug 28.
The renal nerve plexus comprises efferent and afferent fibers. It controls urine production and bodily fluid homeostasis. Efferent fibers to the kidney include sympathetic nerve fibers from their main ganglia, the prevertebral suprarenal ganglia (SrG), and the paravertebral sympathetic chain ganglia (ChG). In the present study, we examined topological innervation from these ganglia to the renal parenchymal segments of the left kidney of the rat. Fluoro-Gold was injected into the rostral or caudal poles of the left kidney. Approximately 50% of the cells in the SrG of rats injected in the rostral pole were labeled, while 60% of the cells in the ChG T13 of rats injected in the caudal pole were labeled. In addition, we performed dual-probe retrograde tracing of the nerves using two kinds of fluorescent-conjugated cholera toxins (f-CTbs) injected into the rostral and caudal poles of the left kidney. The cells labeled with each f-CTb were distributed differently in the left SrG and the lower ChGs; no dual-labeled cells were found in these ganglia. Anterograde tracing with pCAGGS-tdTomato vector transfected into the left SrG showed that tdTomato-labeled nerve varicosities extended to the cortical arterioles and urinary tubules. Immunohistochemistry revealed that they were positive to tyrosine hydroxylase and synaptophysin, suggesting that they possessed sympathetic nerve endings. Our results show that renal efferent nerves in the SrG may control the rostral part of the kidney and innervate the multiple effectors in the cortex. Anat Rec, 300:2263-2272, 2017. © 2017 Wiley Periodicals, Inc.
肾神经丛由传出纤维和传入纤维组成。它控制尿液生成和体液平衡。支配肾脏的传出纤维包括来自其主要神经节，即椎前肾上腺神经节（SrG）和椎旁交感神经链神经节（ChG）的交感神经纤维。在本研究中，我们检查了这些神经节至大鼠左肾实质节段的拓扑神经支配。将荧光金注入左肾的头极或尾极。注入头极的大鼠SrG中约50%的细胞被标记，而注入尾极的大鼠ChG T13中60%的细胞被标记。此外，我们使用两种荧光共轭霍乱毒素（f-CTbs）注入左肾的头极和尾极，对神经进行双探针逆行追踪。用每种f-CTb标记的细胞在左SrG和下ChG中的分布不同；在这些神经节中未发现双标记细胞。将pCAGGS-tdTomato载体转染到左SrG中进行顺行追踪，结果显示tdTomato标记的神经膨体延伸至皮质小动脉和肾小管。免疫组织化学显示它们对酪氨酸羟化酶和突触素呈阳性，表明它们具有交感神经末梢。我们的结果表明，SrG中的肾传出神经可能控制肾脏的头侧部分并支配皮质中的多种效应器。《解剖学记录》，300:2263 - 2272，2017年。© 2017威利期刊公司。
