Department of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
Department of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
Int J Biol Macromol. 2018 Apr 15;110:150-156. doi: 10.1016/j.ijbiomac.2017.08.094. Epub 2017 Aug 20.
Polymicrobial biofilms with fungi and bacteria are the leading cause for the failure of medical devices and related infections. In this study, antibiofilm activities of carboxymethyl chitosan (CM-chitosan) on monomicrobial and polymicrobial biofilms of Staphylococcus epidermidis and Candida tropicalis in vitro were evaluated. CM-chitosan was effective as a sole agent, inhibiting both monomicrobial and polymicrobial biofilms in microplates and also on the silicone surface in short- and long-term periods. Biofilm architecture was investigated by scanning electron microscopy and confocal laser scanning microscopy was used to examine living/dead organisms within biofilm. CM-chitosan inhibited planktonic growth as well as adhesion. Further biofilm formation was inhibited by CM-chitosan added at 90min or 12h after biofilm initiation. CM-chitosan may serve as a possible antibiofilm agent to limit monomicrobial and polymicrobial biofilm.
真菌和细菌的多微生物生物膜是导致医疗器械失效和相关感染的主要原因。在这项研究中，评估了羧甲基壳聚糖（CM-壳聚糖）对表皮葡萄球菌和热带假丝酵母单微生物和多微生物生物膜的体外抗生物膜活性。CM-壳聚糖作为单一试剂是有效的，它不仅可以抑制微板中的单微生物和多微生物生物膜，还可以在短期和长期内在硅树脂表面抑制生物膜。通过扫描电子显微镜研究了生物膜结构，并使用共聚焦激光扫描显微镜检查了生物膜内的活/死生物。CM-壳聚糖抑制浮游生物的生长以及黏附。在生物膜形成 90 分钟或 12 小时后加入 CM-壳聚糖，进一步抑制了生物膜的形成。CM-壳聚糖可能作为一种潜在的抗生物膜剂，限制单微生物和多微生物生物膜的形成。
