Arroyo R, Orozco E
Mol Biochem Parasitol. 1987 Mar;23(2):151-8. doi: 10.1016/0166-6851(87)90150-2.
The adherence of Entamoeba histolytica trophozoites to target cells was studied by using monoclonal antibodies (MAbs) and adhesion-deficient mutants of the parasite. MAbs Adh-1 and Adh-2 reacted with a surface protein of approximately 112 kDa of the total proteins of trophozoites from the wild type strain, clone A, strain HM1:IMSS. Both MAbs reacted weakly with the adhesion-deficient mutant clones, C-98, C-919 and C-923, all derived from HM1:IMSS. MAbs Adh-1 and Adh-2 incubated with trophozoites from clone A inhibited adherence to red blood cells, erythrophagocytosis and cytopathic effect on cell culture monolayers. Antibodies against a approximately 112 kDa polypeptide were found in the sera from patients with hepatic abscess. These results demonstrate that the adherence of trophozoites to target cells is a necessary event in order for cytopathogenicity to occur.
利用单克隆抗体（MAbs）和该寄生虫的黏附缺陷突变体，研究了溶组织内阿米巴滋养体对靶细胞的黏附情况。单克隆抗体Adh-1和Adh-2与来自野生型菌株克隆A（菌株HM1:IMSS）的滋养体总蛋白中一种约112 kDa的表面蛋白发生反应。这两种单克隆抗体与均源自HM1:IMSS的黏附缺陷突变体克隆C-98、C-919和C-923反应较弱。用Adh-1和Adh-2单克隆抗体处理克隆A的滋养体后，可抑制其对红细胞的黏附、红细胞吞噬作用以及对细胞培养单层的细胞病变效应。肝脓肿患者血清中发现了针对约112 kDa多肽的抗体。这些结果表明滋养体对靶细胞的黏附是发生细胞致病性的必要事件。
