Rodríguez M A, Orozco E
J Infect Dis. 1986 Jul;154(1):27-32. doi: 10.1093/infdis/154.1.27.
We have used chemical mutagenesis to isolate 10 independent phagocytosis-deficient mutants of Entamoeba histolytica from a highly virulent clone (strain HM1:IMSS). Phagocytosis-deficient mutants were characterized by several biologic properties related to amoebic pathogenic mechanisms: virulence, adhesion to red blood cells, cytopathic effect of live trophozoites on cell culture monolayers, ability of amoebic extracts to destroy cell culture monolayers, and growth in semisolid agar. All of these mutants were also deficient in in vivo virulence and in vitro cytopathic effect. Three were also deficient in adhesion, and the extracts of three others were unable to destroy cell culture monolayers. These findings suggest a strong correlation between phagocytosis and in vivo and in vitro cytopathogenicity. Both adherence of trophozoites to target cells and toxin activity of crude extracts of amoebae seem to be necessary factors for the expression of the virulence of this parasite. Traits responsible for growth in semisolid agar do not seem to be involved in virulence.
我们利用化学诱变从高毒力克隆株（HM1:IMSS株）中分离出10个独立的溶组织内阿米巴吞噬缺陷型突变体。吞噬缺陷型突变体具有与阿米巴致病机制相关的几种生物学特性：毒力、对红细胞的黏附、活滋养体对细胞培养单层的细胞病变效应、阿米巴提取物破坏细胞培养单层的能力以及在半固体琼脂中的生长情况。所有这些突变体在体内毒力和体外细胞病变效应方面也存在缺陷。其中三个在黏附方面也有缺陷，另外三个的提取物无法破坏细胞培养单层。这些发现表明吞噬作用与体内和体外细胞致病性之间存在很强的相关性。滋养体对靶细胞的黏附和阿米巴粗提物的毒素活性似乎都是该寄生虫表达毒力的必要因素。与在半固体琼脂中生长相关的特性似乎与毒力无关。
