Kavaliers M
Peptides. 1987 Jan-Feb;8(1):1-5. doi: 10.1016/0196-9781(87)90155-0.
The effects of prolyl-leucyl-glycinamide (MIF-1, PLG), tyrosine-prolyl-leucyl-glycinamide (Tyr-MIF-1, YPLG) and naloxone on morphine and warm and cold stress-induced increases in the latency of the thermal (40 degrees C hot plate) avoidance behaviors of the terrestrial snail, Cepaea nemoralis, were examined. All three substances blocked the morphine- and warm stress-induced opioid analgesia, while having no effects on non-opioid cold stress-induced analgesia. Tyr-MIF-1 had a significantly greater inhibitory effect than MIF-1. These results indicate that MIF-1 and Tyr-MIF-1 antagonize the antinociceptive effects of exogenous opiates and opioid-mediated analgesia in snails in a manner analogous to that described for mammals. This raises the possibility of an evolutionary conservation of functional opioid antagonists.
研究了脯氨酰 - 亮氨酰 - 甘氨酰胺（MIF - 1，PLG）、酪氨酸 - 脯氨酰 - 亮氨酰 - 甘氨酰胺（Tyr - MIF - 1，YPLG）和纳洛酮对吗啡以及温热和冷应激诱导的陆生蜗牛（野蛞蝓）热（40摄氏度热板）回避行为潜伏期增加的影响。这三种物质均阻断了吗啡和温热应激诱导的阿片类镇痛作用，而对非阿片类冷应激诱导的镇痛作用无影响。Tyr - MIF - 1的抑制作用明显强于MIF - 1。这些结果表明，MIF - 1和Tyr - MIF - 1以类似于哺乳动物的方式拮抗外源性阿片类药物的镇痛作用以及蜗牛中阿片类介导的镇痛作用。这增加了功能性阿片类拮抗剂进化保守性的可能性。
