Ristic Natasa, Ajdzanovic Vladimir, Manojlovic-Stojanoski Milica, Maliković Jovana, Uscebrka Gordana, Markovic Zorica, Milosevic Verica
Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
Cell J. 2017 Oct;19(3):461-468. doi: 10.22074/cellj.2017.4334. Epub 2017 Aug 19.
Estrogen replacement therapy remains current as a therapeutic approach to treat menopausal symptoms and may significantly affect hormone-producing cells in the female pituitaries. The aim of this study was to examine the histological parameters of pituitary mammotrophs and prolactin secretion after chronic estradiol treatment in ovariectomized adult female rats, reflecting premature menopause.
In this experimental study, adult female Wistar rats were divided into non-ovariectomized (C), ovariectomized (OVX) and estradiol-treated ovariectomized (OVX+E) groups. Estradiol dipropionate [0.625 mg/kg body mass per day] was administered for four weeks, while the C and OVX groups received vehicle alone. Mammotrophs were identified by the peroxidase-antiperoxidase (PAP) immunohistochemical procedure, while prolactin concentrations were measured by the non-isotopic two-step assay (Delfia) method. Comparison of the differences between groups was performed using one-way analysis of variance (ANOVA) and Tukay (honest significant difference) HSD test.
Ovariectomy caused significant (P<0.05) decreases in mammotroph optical density (OD), volume density (VV) and number per mm2 by 29, 27 and 34%, respectively, in comparison with the C females. In the OVX+E group, significant (P<0.05) increases in OD, cell volume, VV and number of mammotrophs per mm2 by 181, 15%, 5.8-fold and 5.2-fold, respectively, were observed when compared to OVX animals. The serum prolactin concentration in OVX females was significantly (P<0.05) decreased by 14% in comparison to the C group, while in OVX+E females, prolactin levels were significantly (P<0.05) increased by 53% compared to the OVX controls.
Estradiol supplementation in ovariectomized females is followed by stimulatory histological and secretory changes of the mammotrophs. These results could serve as indicators of possible prolactinome development upon estradiol application in premature menopausal subjects.
雌激素替代疗法仍是治疗更年期症状的一种常用治疗方法，且可能会对女性垂体中产生激素的细胞产生显著影响。本研究的目的是检测去卵巢成年雌性大鼠（反映过早绝经）经慢性雌二醇治疗后垂体催乳素细胞的组织学参数及催乳素分泌情况。
在本实验研究中，成年雌性Wistar大鼠被分为未去卵巢组（C组）、去卵巢组（OVX组）和雌二醇治疗去卵巢组（OVX + E组）。每天给予二丙酸雌二醇[0.625 mg/kg体重]，持续四周，而C组和OVX组仅给予赋形剂。采用过氧化物酶 - 抗过氧化物酶（PAP）免疫组织化学方法鉴定催乳素细胞，采用非同位素两步法（Delfia）测定催乳素浓度。使用单因素方差分析（ANOVA）和图基（诚实显著差异）HSD检验对组间差异进行比较。
与C组雌性大鼠相比，去卵巢导致催乳素细胞光密度（OD）、体积密度（VV）和每平方毫米数量分别显著降低（P < 0.05）29%、27%和34%。与OVX组动物相比，在OVX + E组中，观察到OD、细胞体积、VV和每平方毫米催乳素细胞数量分别显著增加（P < 0.05）181%、15%、5.8倍和5.2倍。与C组相比，OVX雌性大鼠血清催乳素浓度显著降低（P < 0.05）14%，而与OVX对照组相比，OVX + E雌性大鼠催乳素水平显著增加（P < 0.05）53%。
去卵巢雌性大鼠补充雌二醇后，催乳素细胞会出现刺激性的组织学和分泌变化。这些结果可作为绝经过早的受试者应用雌二醇后可能发生催乳素瘤的指标。
