Biotechnology of Horticultural Crops, Technische Universität München, Liesel-Beckmann-Straße 1, 85354, Freising-Weihenstephan, Germany.
Department of Crop Production and Protection, Obafemi Awolowo University, PMB 013, Ilé-Ifè, 220005, Nigeria.
Phytochem Anal. 2018 Jan;29(1):48-58. doi: 10.1002/pca.2713. Epub 2017 Aug 24.
Pyrrolizidine alkaloids (PAs) are secondary plant metabolites with considerable hepatoxic, tumorigenic and genotoxic potential. For separation, reversed phase chromatography is commonly used because of its excellent compatibility with detection by mass spectrometry. However, reversed phase chromatography has a low selectivity for PAs.
The objective of this work was to investigate the suitability of cation exchange chromatography for separation of PAs and to develop a rapid method for quantification of jacobine in Crassocephalum crepidioides that is suitable for analysis of huge sample numbers as required for mutant screening procedures.
We demonstrate that cation exchange chromatography offers excellent selectivity for PAs allowing their separation from most other plant metabolites. Due to the high selectivity, plant extracts can be directly analysed after simple sample preparation. Detection with UV at 200 nm instead of mass spectrometry can be applied, which makes the method very simple and cost-effective. The recovery rate of the method exceeded 95%, the intra-day and inter-day standard deviations were below 7% and the limit of detection and quantification were 1 mg/kg and 3 mg/kg, respectively.
The developed method is sufficiently sensitive for reproducible detection of jacobine in C. crepidioides. Simple sample preparation and rapid separation allows for quantification of jacobine in plant material in a high-throughput manner. Thus, the method is suitable for genetic screenings and may be applicable for other plant species, for instance Jacobaea maritima. In addition, our results show that C. crepidioides cannot be considered safe for human consumption. Copyright © 2017 John Wiley & Sons, Ltd.
吡咯里西啶生物碱（PAs）是具有相当肝毒性、致癌性和遗传毒性的次生植物代谢物。由于与质谱检测具有极好的兼容性，反相色谱通常用于分离。然而，反相色谱对 PAs 的选择性较低。
本工作旨在研究阳离子交换色谱分离 PAs 的适用性，并建立一种快速测定白花丹中 Jacobine 的方法，该方法适用于突变筛选过程中所需的大量样品分析。
我们证明阳离子交换色谱对 PAs 具有极好的选择性，允许它们与大多数其他植物代谢物分离。由于高选择性，植物提取物在简单的样品制备后可以直接分析。与质谱检测相比，可以应用于 200nm 处的紫外检测，这使得该方法非常简单且具有成本效益。该方法的回收率超过 95%，日内和日间标准偏差低于 7%，检测限和定量限分别为 1mg/kg 和 3mg/kg。
所开发的方法足以灵敏地检测白花丹中的 Jacobine。简单的样品制备和快速分离允许高通量地定量植物材料中的 Jacobine。因此，该方法适用于遗传筛选，也可能适用于其他植物物种，例如 Jacobaea maritima。此外，我们的结果表明白花丹不能被认为是人类食用的安全植物。版权所有 © 2017 约翰威立父子公司
