Hatier R, Grignon G
Virchows Arch B Cell Pathol Incl Mol Pathol. 1986;52(4):305-18. doi: 10.1007/BF02889973.
Cryptorchidism was simulated in 13-15-day-old rats by severing the gubernaculum testis and fixing the testis to the abdominal wall. Ultrastructural examination of the testis was made 100 days after birth when a number of modifications to the seminiferous tubules were noted. Germ cells were scanty, with only occasional spermatogonia and primary spermatocytes persisting. The nuclei of Sertoli cells were regular and oval or indented in shape. Their cytoplasm was characterized by a rich smooth endoplasmic reticulum, lipid inclusions and mitochondria with tubulo-vesicular cristae indicative of steroïdogenic activity. The decrease in the number of the germ cells induced a membrane rearrangement with numerous tight junctions and interdigitations between the Sertoli cells. Sertoli cell-specific junctional complexes were very extensive. The lamina propria of the seminiferous tubule appeared thickened and folded and the multilayered basal lamina had complex folds. After fixation with glutaraldehyde containing lanthanum, the latter substance was identified in the basal intercellular spaces of the seminiferous tubules indicating that the blood-testis barrier remains functional in the intra-abdominal testis.
通过切断睾丸引带并将睾丸固定于腹壁，在13 - 15日龄大鼠中模拟隐睾症。出生100天后对睾丸进行超微结构检查，此时观察到生精小管有一些改变。生殖细胞稀少，仅偶尔可见精原细胞和初级精母细胞留存。支持细胞的细胞核形状规则，呈椭圆形或有凹陷。其细胞质的特征是富含滑面内质网、脂滴和具有管状嵴的线粒体，提示有类固醇生成活性。生殖细胞数量的减少导致支持细胞之间出现大量紧密连接和指状交叉的膜重排。支持细胞特异性连接复合体非常广泛。生精小管的固有层增厚并折叠，多层基底膜有复杂的褶皱。用含镧的戊二醛固定后，在生精小管的基底细胞间隙中发现了镧，表明血睾屏障在腹腔内睾丸中仍发挥功能。
