Donchenko Ekaterina K, Pechnikova Evgeniya V, Mishyna Maryia Yu, Manukhova Tatiana I, Sokolova Olga S, Nikitin Nikolai A, Atabekov Joseph G, Karpova Olga V
Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
Laboratory of Electron Microscopy, V.A. Shoubnikov Institute of Crystallography of Russian Academy of Sciences, Moscow, Russia.
PLoS One. 2017 Aug 24;12(8):e0183824. doi: 10.1371/journal.pone.0183824. eCollection 2017.
Plant viruses and their virus-like particles (VLPs) have a lot of advantages for biotechnological applications including complete safety for humans. Alternanthera mosaic virus (AltMV) is a potentially promising object for design of novel materials. The 3D structures of AltMV virions and its VLPs were obtained by single particle EM at ~13Å resolution. The comparison of the reconstructions and a trypsin treatment revealed that AltMV CPs possesses a different fold in the presence (virions) and absence of viral RNA (VLPs). For the first time, the structure of morphologically similar virions and virus-like particles based on the coat protein of a helical filamentous plant virus is shown to be different. Despite this, both AltMV virions and VLPs are stable in a wide range of conditions. To provide a large amount of AltMV for biotechnology usage the isolation procedure was modified.
植物病毒及其病毒样颗粒（VLP）在生物技术应用方面具有诸多优势，包括对人类完全安全。Alternanthera花叶病毒（AltMV）是设计新型材料的一个潜在有前景的对象。通过单颗粒电子显微镜在约13埃分辨率下获得了AltMV病毒粒子及其VLP的三维结构。重建结果的比较以及胰蛋白酶处理表明，AltMV衣壳蛋白在存在病毒RNA（病毒粒子）和不存在病毒RNA（VLP）的情况下具有不同的折叠方式。首次表明，基于螺旋丝状植物病毒衣壳蛋白的形态相似的病毒粒子和病毒样颗粒的结构是不同的。尽管如此，AltMV病毒粒子和VLP在广泛的条件下都是稳定的。为了为生物技术用途提供大量的AltMV，对分离程序进行了改进。
