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莲子草花叶病毒及其外壳蛋白的特性分析

Characterization of Alternanthera mosaic virus and its Coat Protein.

作者信息

Mukhamedzhanova Anna A, Smirnov Alexander A, Arkhipenko Marina V, Ivanov Peter A, Chirkov Sergey N, Rodionova Nina P, Karpova Olga V, Atabekov Joseph G

机构信息

Department of Virology, Moscow State University, Moscow 119991, Russia.

出版信息

Open Virol J. 2011;5:136-40. doi: 10.2174/1874357901105010136. Epub 2011 Nov 21.

DOI:10.2174/1874357901105010136
PMID:22216073
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3245411/
Abstract

A new isolate of Alternantheramosaic virus (AltMV-MU) was purified from Portulaca grandiflora plants. It has been shown that the AltMV-MU coat protein (CP) can be efficiently reassembled in vitro under different conditions into helical RNA-free virus-like particles (VLPs) antigenically related to native virus. The AltMV-MU and VLPs were examined by atomic force and transmission electron microscopies. The encapsidated AltMV-MU RNA is nontranslatable in vitro. However, it can be translationally activated by CP phosphorylation or by binding to the TGB1protein from the virus-coded movement triple gene block.

摘要

从大花马齿苋植物中纯化出一种新的锦绣苋花叶病毒分离株（AltMV-MU）。研究表明，AltMV-MU外壳蛋白（CP）在不同条件下可在体外有效重组成与天然病毒具有抗原相关性的无RNA螺旋状病毒样颗粒（VLP）。通过原子力显微镜和透射电子显微镜对AltMV-MU和VLP进行了检测。包裹在衣壳中的AltMV-MU RNA在体外不可翻译。然而，它可以通过CP磷酸化或与病毒编码的运动三联基因块中的TGB1蛋白结合而被翻译激活。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0b8/3245411/b2f2ff9338c5/TOVJ-5-136_F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0b8/3245411/57fb3062b960/TOVJ-5-136_F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0b8/3245411/98f4efeaa972/TOVJ-5-136_F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0b8/3245411/9b5d32e0c0c1/TOVJ-5-136_F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0b8/3245411/b2f2ff9338c5/TOVJ-5-136_F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0b8/3245411/57fb3062b960/TOVJ-5-136_F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0b8/3245411/98f4efeaa972/TOVJ-5-136_F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0b8/3245411/9b5d32e0c0c1/TOVJ-5-136_F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0b8/3245411/b2f2ff9338c5/TOVJ-5-136_F4.jpg

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Family Flexiviridae: a case study in virion and genome plasticity.柔线病毒科：病毒粒子与基因组可塑性的一个案例研究
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Immunogenicity of papaya mosaic virus-like particles fused to a hepatitis C virus epitope: evidence for the critical function of multimerization.
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