Future Industries Institute, University of South Australia, Mawson Lakes Campus, Mawson Lakes, SA 5095, Australia; School of Information Technology and Mathematical Sciences, University of South Australia, Mawson Lakes Campus, Mawson Lakes, SA 5095, Australia.
Future Industries Institute, University of South Australia, Mawson Lakes Campus, Mawson Lakes, SA 5095, Australia; School of Information Technology and Mathematical Sciences, University of South Australia, Mawson Lakes Campus, Mawson Lakes, SA 5095, Australia.
Colloids Surf B Biointerfaces. 2017 Nov 1;159:468-476. doi: 10.1016/j.colsurfb.2017.08.005. Epub 2017 Aug 4.
A polyelectrolyte multilayer (PEM) system based on biopolymers has been constructed and studied in its formation and enzymatic breakdown. The multilayer is composed of fucoidan (a proven antimicrobial/anti-inflammatory seaweed-based polysaccharide) and poly-l-arginine (a polypeptide that can be readily degraded with trypsin to yield arginine, a known NO donor), thus making the multilayer a potential dual action surface treatment for wound dressings. Studies on the formation of the multilayer revealed that the film built-up in the expected stepwise manner with consistent reversal of the zeta potential upon the adsorption of each subsequent polyion. The completed film (8 bilayers) was seen to have low hydration (30% water), as determined by HO/DO solvent replacement studies using the quartz crystal microbalance, with an adsorbed mass (without hydration water) of approx. 4.8μgcm, as determined by quantitative attenuated total reflectance Fourier transform infrared (ATR FTIR) spectroscopy. The enzymatic breakdown of the film in response to exposure to trypsin was also investigated, and the film was seen to release both polymers over time, with a projected complete film removal period of approximately 24h. Critically, this information was determined using ATR FTIR spectroscopy experiments, which allowed unambiguous deconvolution of the removal rates of the two polyions, which is information that cannot be obtained from other methodologies used to study enzymatic breakdown of surface films.
已经构建并研究了基于生物聚合物的聚电解质多层（PEM）系统，以研究其形成和酶促分解。该多层由褐藻酸盐（一种已被证明具有抗菌/抗炎作用的海藻多糖）和聚精氨酸（一种可被胰蛋白酶快速降解以产生精氨酸的多肽，精氨酸是已知的一氧化氮供体）组成，因此使多层成为潜在的双重作用伤口敷料表面处理方法。对多层形成的研究表明，薄膜以预期的逐步方式构建，并且在吸附每个后续聚离子时，zeta 电位一致反转。通过使用石英晶体微天平进行 HO/DO 溶剂置换研究，确定完成的薄膜（8 个双层）具有低水合作用（30%水），而通过定量衰减全反射傅里叶变换红外（ATR FTIR）光谱法确定的吸附质量（无水合水）约为 4.8μgcm。还研究了薄膜对胰蛋白酶暴露时的酶促分解，并且随着时间的推移，观察到两种聚合物都从薄膜中释放出来，预计完全去除薄膜的时间约为 24 小时。至关重要的是，这一信息是通过 ATR FTIR 光谱实验确定的，该实验允许明确区分两种聚离子的去除速率，而这是无法从用于研究表面膜酶促分解的其他方法获得的信息。
