Preparation and Bioactivity of Exopolysaccharide from an Endophytic Fungus sp. of the Medicinal Plant Gynostemma Pentaphylla.

BACKGROUND

Many exopolysaccharides from the endophytes in medicinal plants possess various potential bioactivities.

MATERIALS AND METHODS

The endophytic fungus JY25 was isolated from the leave of the Chinese medicinal plant and identified as sp. by its phylogenetic and physiological analysis. One exopolysaccharide (EPS) fraction was isolated from the fermentation broth by ethanol precipitation and purified by gel filtration chromatography on Sepharose CL-6B. The molecular characteristics were examined by GC-MS, FT-IR, and multiangle laser light scattering (MALLS).

RESULTS

The monosaccharide composition analysis indicated that the purified EPS was mainly composed of glucose, mannose, arabinose, and galactose with the molecular ratio of 78.29: 8.99: 8.64: 4.08. FT-IR spectral analysis of the purified EPS revealed prominent characteristic groups, such as carbonyl bond, pyranose ring, and so on. The weight-average molar mass and the polydispersity ratio of the EPS were revealed to be 1.961×10 g/mol and 1.838, respectively. Furthermore, thermo gravimetric analysis (TGA) indicated that the degradation temperature of the purified EPS was 305 C. The purified EPS from the endophytic fungus sp. displayed antioxidant and antiproliferative activities.

CONCLUSION

The results demonstrated that the EPS could be used as a healthful food and material source in pharmaceutical industries.

SUMMARY

An exopolysaccharides (EPS) with antioxidant and antiproliferative activities from an endophytic fungus sp. was reported. ANOVA: Analysis of variance; DPPH: 2,2-diphenyl-1-picrylhydrazyl; EPS: Exopolysaccharide; FT-IR: Fourier transform infrared spectroscopy; GC-MS: Gas chromatography-mass spectrometry; Mw: Mass weight; MALLS: Multiangle laser light scattering; SEC: Size Exclusion Chomatography; SPSS: Statistical Package of the Social Science; TGA: Thermo gravimetric analysis; TFA: Trifluoroacetic acid.