Zhang Huiru, Wang Xueqin, Li Ruifang, Sun Xincheng, Sun Siwen, Li Qiang, Xu Chunping
College of Bioengineering, Henan University of Technology, Zhengzhou, Henan, P. R. China.
College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, P. R. China.
Pharmacogn Mag. 2017 Jul-Sep;13(51):477-482. doi: 10.4103/0973-1296.211033. Epub 2017 Jul 19.
Many exopolysaccharides from the endophytes in medicinal plants possess various potential bioactivities.
The endophytic fungus JY25 was isolated from the leave of the Chinese medicinal plant and identified as sp. by its phylogenetic and physiological analysis. One exopolysaccharide (EPS) fraction was isolated from the fermentation broth by ethanol precipitation and purified by gel filtration chromatography on Sepharose CL-6B. The molecular characteristics were examined by GC-MS, FT-IR, and multiangle laser light scattering (MALLS).
The monosaccharide composition analysis indicated that the purified EPS was mainly composed of glucose, mannose, arabinose, and galactose with the molecular ratio of 78.29: 8.99: 8.64: 4.08. FT-IR spectral analysis of the purified EPS revealed prominent characteristic groups, such as carbonyl bond, pyranose ring, and so on. The weight-average molar mass and the polydispersity ratio of the EPS were revealed to be 1.961×10 g/mol and 1.838, respectively. Furthermore, thermo gravimetric analysis (TGA) indicated that the degradation temperature of the purified EPS was 305 C. The purified EPS from the endophytic fungus sp. displayed antioxidant and antiproliferative activities.
The results demonstrated that the EPS could be used as a healthful food and material source in pharmaceutical industries.
An exopolysaccharides (EPS) with antioxidant and antiproliferative activities from an endophytic fungus sp. was reported. ANOVA: Analysis of variance; DPPH: 2,2-diphenyl-1-picrylhydrazyl; EPS: Exopolysaccharide; FT-IR: Fourier transform infrared spectroscopy; GC-MS: Gas chromatography-mass spectrometry; Mw: Mass weight; MALLS: Multiangle laser light scattering; SEC: Size Exclusion Chomatography; SPSS: Statistical Package of the Social Science; TGA: Thermo gravimetric analysis; TFA: Trifluoroacetic acid.
许多来自药用植物内生菌的胞外多糖具有多种潜在生物活性。
从该中药植物叶片中分离出内生真菌JY25，并通过系统发育和生理分析将其鉴定为 属真菌。通过乙醇沉淀从发酵液中分离出一种胞外多糖（EPS）组分，并在Sepharose CL - 6B上进行凝胶过滤色谱纯化。通过气相色谱 - 质谱联用（GC - MS）、傅里叶变换红外光谱（FT - IR）和多角度激光光散射（MALLS）对其分子特征进行检测。
单糖组成分析表明，纯化后的EPS主要由葡萄糖、甘露糖、阿拉伯糖和半乳糖组成，分子比例为78.29 : 8.99 : 8.64 : 4.08。纯化后的EPS的FT - IR光谱分析显示出突出的特征基团，如羰基键、吡喃糖环等。EPS的重均摩尔质量和多分散比分别为1.961×10 g/mol和1.838。此外，热重分析（TGA）表明纯化后的EPS的降解温度为305℃。从 属内生真菌中纯化得到的EPS具有抗氧化和抗增殖活性。
结果表明，该EPS可作为保健食品和制药工业的原料来源。
报道了一种来自 属内生真菌的具有抗氧化和抗增殖活性的胞外多糖（EPS）。ANOVA：方差分析；DPPH：2,2 - 二苯基 - 1 - 苦基肼；EPS：胞外多糖；FT - IR：傅里叶变换红外光谱；GC - MS：气相色谱 - 质谱联用；Mw：质量重量；MALLS：多角度激光光散射；SEC：尺寸排阻色谱；SPSS：社会科学统计软件包；TGA：热重分析；TFA：三氟乙酸。
