Ogidi Clement Olusola, Ubaru Adaeze Mascot, Ladi-Lawal Temilayo, Thonda Oluwakemi Abike, Aladejana Oluwatoyin Modupe, Malomo Olu
Biotechnology Unit, Department of Biological Sciences, Kings University, PMB 555, Odeomu, Nigeria.
Microbiology Unit, Department of Biological Sciences, Kings University, PMB 555, Odeomu, Nigeria.
Heliyon. 2020 Dec 10;6(12):e05685. doi: 10.1016/j.heliyon.2020.e05685. eCollection 2020 Dec.
spp. are white-rot fungi that utilize different agro-wastes to produce useful biologically active compounds. In this study, exopolysaccharides (EPS) were produced by in submerged culture supplemented with different agro-wastes. Functional groups in EPS were revealed using Fourier Transform-Infrared (FT-IR) spectroscopy. Antimicrobial activity of EPS was tested against microorganisms using agar well diffusion. Scavenging potentials of EPS was tested against 1, 1- diphenyl-2-picryhydrazyl (DPPH), hydroxyl (OH), iron (Fe) and nitric oxide (NO) radicals. prebiotic activity of EPS was carried out. The highest yield (5.60 g/L) of EPS was produced by in submerged culture supplemented with groundnut shell (20.0 g/L). The functional groups in EPS were hydroxyl (-OH), methyl (-CH), ketone (-RCOH) and carbonyl group (-C=O). EPS displayed zones of inhibition (5.00-14.00 mm) against tested microorganisms. Scavenging activity of EPS ranged from 65.70-81.80% against DPPH. EPS supported the growth of and with values ranged from 3.04 × 10-3.40 × 10 cfu/ml and 2.50 × 10-2.81 × 10 cfu/ml, respectively. Submerged culture of with addition of agro-wastes enhanced yield of EPS. The EPS exhibited bio-functional properties like antimicrobial, antioxidant and prebiotic activities. Hence, agrowastes can be recycled in submerged fermentation with fungi to produce promising biomaterials for biopharmaceutical applications.
某些物种是白腐真菌，它们利用不同的农业废弃物来生产有用的生物活性化合物。在本研究中，在添加不同农业废弃物的深层培养中，由[具体菌种名称未给出]产生了胞外多糖（EPS）。使用傅里叶变换红外（FT - IR）光谱揭示了EPS中的官能团。使用琼脂孔扩散法测试了EPS对微生物的抗菌活性。测试了EPS对1,1 - 二苯基 - 2 - 苦基肼（DPPH）、羟基（OH）、铁（Fe）和一氧化氮（NO）自由基的清除潜力。进行了EPS的益生元活性研究。在添加花生壳（20.0 g/L）的深层培养中，[具体菌种名称未给出]产生的EPS产量最高（5.60 g/L）。EPS中的官能团为羟基（-OH）、甲基（-CH）、酮基（-RCOH）和羰基（-C = O）。EPS对测试微生物显示出抑制圈（5.00 - 14.00 mm）。EPS对DPPH的清除活性范围为65.70 - 81.80%。EPS支持[具体菌种名称未给出]和[具体菌种名称未给出]的生长，其值分别为3.04×10 - 3.40×10 cfu/ml和2.50×10 - 2.81×10 cfu/ml。添加农业废弃物的[具体菌种名称未给出]深层培养提高了EPS的产量。EPS表现出抗菌、抗氧化和益生元活性等生物功能特性。因此，农业废弃物可以在真菌深层发酵中循环利用，以生产用于生物制药应用的有前景的生物材料。
