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非编码RNA在实验性放线菌病中表达存在差异。

Non-Coding RNAs are Differentially Expressed by and in Experimental Actinomycetoma.

作者信息

Cruz-Rabadán Josué S, Miranda-Ríos Juan, Espín-Ocampo Guadalupe, Méndez-Tovar Luis J, Maya-Pineda Héctor Rubén, Hernández-Hernández Francisca

机构信息

Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Ciudad de México, México.

Unidad de Genética de la Nutrición, Instituto Nacional de Pediatría and Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, UNAM, Ciudad de México, México.

出版信息

Open Microbiol J. 2017 Jun 30;11:112-125. doi: 10.2174/1874285801711010112. eCollection 2017.

DOI:10.2174/1874285801711010112
PMID:28839491
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5543724/
Abstract

INTRODUCTION

spp. are common soil-inhabiting bacteria that frequently infect humans through traumatic injuries or inhalation routes and cause infections, such as actinomycetoma and nocardiosis, respectively. is the main aetiological agent of actinomycetoma in various countries. Many bacterial non-coding RNAs are regulators of genes associated with virulence factors.

OBJECTIVE

The aim of this work was to identify non-coding RNAs (ncRNAs) expressed during infection conditions and in free-living form () in .

METHODS AND RESULT

The transcriptome (predominately < 200 nucleotides) was determined by RNA next-generation sequencing in both conditions. A total of seventy ncRNAs were identified in both conditions. Among these ncRNAs, 18 were differentially expressed, 12 were located within intergenic regions, and 2 were encoded as antisense of 2 different genes. Finally, 10 of these ncRNAs were studied by rapid amplification of cDNA ends and/or quantitative reverse transcription polymerase chain reaction. Interestingly, 3 transcripts corresponded to tRNA-derived fragments (tRNAs), and one transcript was overlapped between an intergenic region and the 5´end of the 23S rRNA. Expression of these last four transcripts was increased during infection compared with the conditions.

CONCLUSION

The results of this work suggest a possible role for these transcripts in the regulation of virulence genes in actinomycetoma pathogenesis.

摘要

引言

诺卡氏菌属是常见的土壤栖息细菌，常通过创伤或吸入途径感染人类并分别引起放线菌瘤和诺卡氏菌病等感染。在各个国家，诺卡氏菌是放线菌瘤的主要病原体。许多细菌非编码RNA是与毒力因子相关基因的调节因子。

目的

本研究旨在鉴定在感染条件下以及在诺卡氏菌自由生活形式（体外）中表达的非编码RNA（ncRNA）。

方法与结果

通过RNA下一代测序在两种条件下测定诺卡氏菌转录组（主要<200个核苷酸）。在两种条件下共鉴定出70种ncRNA。在这些ncRNA中，18种差异表达，12种位于基因间区域，2种编码为2个不同基因的反义RNA。最后，通过cDNA末端快速扩增和/或定量逆转录聚合酶链反应研究了其中10种ncRNA。有趣的是，3个转录本对应于tRNA衍生片段（tRF），一个转录本在基因间区域和23S rRNA的5´端之间重叠。与体外条件相比，在诺卡氏菌感染期间这最后四个转录本的表达增加。

结论

本研究结果表明这些转录本在放线菌瘤发病机制中对毒力基因的调节可能起作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19fb/5543724/f7a4ea625153/TOMICROJ-11-112_F6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19fb/5543724/74b4b492354e/TOMICROJ-11-112_F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19fb/5543724/fd3d6c632323/TOMICROJ-11-112_F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19fb/5543724/3a4504020dc5/TOMICROJ-11-112_F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19fb/5543724/00607778dcf2/TOMICROJ-11-112_F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19fb/5543724/3e6129a2bb42/TOMICROJ-11-112_F5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19fb/5543724/f7a4ea625153/TOMICROJ-11-112_F6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19fb/5543724/74b4b492354e/TOMICROJ-11-112_F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19fb/5543724/fd3d6c632323/TOMICROJ-11-112_F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19fb/5543724/3a4504020dc5/TOMICROJ-11-112_F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19fb/5543724/00607778dcf2/TOMICROJ-11-112_F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19fb/5543724/3e6129a2bb42/TOMICROJ-11-112_F5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19fb/5543724/f7a4ea625153/TOMICROJ-11-112_F6.jpg

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