Paredes-Amaya Claudia C, Manzano-Gayosso Patricia, Hernández-Hernández Francisca
Departamento de Microbiología, Escuela de Ciencias Básicas, Facultad de Salud, Universidad del Valle, Cali, Colombia.
Departamento de Ciencias Básicas de la Salud, Facultad de Ciencias de la Salud, Pontificia Universidad Javeriana Cali, Cali, Colombia.
Curr Microbiol. 2022 Oct 6;79(11):335. doi: 10.1007/s00284-022-03028-8.
Sex steroid hormones have an important physiological role in humans. They can also affect the gene expression of many organisms, including bacteria. In Mexico, Nocardia brasiliensis is the main causative agent of actinomycetoma, a granulomatous disease more frequent in men than women, which is thought to be related to a higher occupational risk in men. Therefore, it has been suggested that differences in clinical presentation could be related to sex steroid hormone levels. Attempting to explain the differences in actinomycetoma prevalence between men and women, in this work, the effect of progesterone and dihydrotestosterone on the genetic expression of N. brasiliensis was investigated using a differential display polymerase chain reaction assay. The results showed that both hormones affected the expression of genes encoding proteins related to central metabolism and hypothetical proteins with unknown functions. This study also demonstrated the utility of differential display in this modern era and provided a first approach to the effect of sex hormones on N. brasiliensis gene expression.
性类固醇激素在人类中具有重要的生理作用。它们还能影响包括细菌在内的许多生物体的基因表达。在墨西哥，巴西诺卡菌是放线菌性足菌肿的主要病原体，这种肉芽肿性疾病在男性中比女性更常见，据认为这与男性较高的职业风险有关。因此，有人提出临床表现的差异可能与性类固醇激素水平有关。为了解释男性和女性放线菌性足菌肿患病率的差异，在这项研究中，使用差异显示聚合酶链反应分析法研究了孕酮和双氢睾酮对巴西诺卡菌基因表达的影响。结果表明，这两种激素均影响与中心代谢相关的蛋白质编码基因以及功能未知的假定蛋白质的表达。这项研究还证明了差异显示在当代的实用性，并首次探讨了性激素对巴西诺卡菌基因表达的影响。
