Kawano Michihiro, Itomine Ichiro, Monma Masahiko, Asakawa Kazumi, Toyoda Atsushi, Miyaguchi Yuji
Department of Nursing, School of Health Sciences, Ibaraki Prefectural University of Health Sciences, Ibaraki, Japan.
Department of Radiological Sciences, School of Health Sciences, Ibaraki Prefectural University of Health Sciences, Ibaraki, Japan.
SAGE Open Med. 2017 Aug 14;5:2050312117725643. doi: 10.1177/2050312117725643. eCollection 2017.
Using ovariectomized rats, we examined the influence of combined exercise tolerance and natto intake on the bone loss inhibitory effect.
We divided female Wistar rats into the following groups: Ovariectomy, Ovariectomy + Exercise, Ovariectomy + Natto Intake, Ovariectomy + Exercise + Natto Intake, and Pseudo-operative (Sham group). After conducting experiments on each group, we collected the tissues and performed morphological and molecular biological analyses.
In comparison with the Ovariectomy group, only in the Ovariectomy + Exercise group was there a significant bone loss inhibitory effect in the femoral cancellous bone. Although there was a tendency toward this trend seen in the Natto Intake and Exercise + Natto Intake groups, these differences were not significant. The increase in messenger RNA expression levels of alkaline phosphatase (osteoblast marker) in the bone marrow caused by ovariectomy was suppressed by individual factors, and by those in combination. However, messenger RNA expression levels of estrogen receptor alpha in the bone marrow showed a decreasing tendency with each factor, and decreased significantly with the combination, similar to the Sham group.
This suggests that natto intake and exercise maintain bone mass by different molecular mechanisms and that these two factors do not simply act synergistically in combination to maintain bone mass.
我们使用去卵巢大鼠，研究了运动耐力与纳豆摄入相结合对骨丢失抑制作用的影响。
我们将雌性Wistar大鼠分为以下几组：去卵巢组、去卵巢+运动组、去卵巢+纳豆摄入组、去卵巢+运动+纳豆摄入组和假手术组（假手术组）。对每组进行实验后，我们收集组织并进行形态学和分子生物学分析。
与去卵巢组相比，仅去卵巢+运动组在股骨松质骨中有显著的骨丢失抑制作用。虽然在纳豆摄入组和运动+纳豆摄入组中也有这种趋势，但这些差异不显著。去卵巢引起的骨髓中碱性磷酸酶（成骨细胞标志物）信使核糖核酸表达水平的升高受到单一因素以及这些因素组合的抑制。然而，骨髓中雌激素受体α的信使核糖核酸表达水平随每个因素呈下降趋势，并且与假手术组相似，在因素组合时显著下降。
这表明纳豆摄入和运动通过不同的分子机制维持骨量，并且这两个因素并非简单地协同作用以维持骨量。
