Urquhart Andrew S, Idnurm Alexander
School of BioSciences, University of Melbourne, Parkville, VIC, 3010, Australia.
Curr Microbiol. 2017 Dec;74(12):1438-1446. doi: 10.1007/s00284-017-1338-3. Epub 2017 Aug 24.
An insertional mutant with reduced pathogenicity on Brassica napus was identified in the plant pathogenic fungus Leptosphaeria maculans. The transfer-DNA molecule from Agrobacterium tumefaciens inserted into a gene encoding a protein with similarity to Sit4-associated proteins (SAPs). In contrast to Saccharomyces cerevisiae which has four members of the SAP family, there is a single copy of the gene in L. maculans. The mutant had normal spore production and spore germination, but altered hyphal branching, suggesting that nutrient signaling is impaired in the strain. This is the first time that a SAP gene has been mutated in a filamentous fungus and links the function of SAP proteins to plant pathogenesis and hyphal branching.
在植物病原真菌大茎点菌中鉴定出了一个对甘蓝型油菜致病性降低的插入突变体。根癌农杆菌的转移DNA分子插入到了一个编码与Sit4相关蛋白（SAPs）相似的蛋白质的基因中。与酿酒酵母有四个SAP家族成员不同，大茎点菌中该基因只有一个拷贝。该突变体孢子产生和孢子萌发正常，但菌丝分支改变，这表明该菌株的营养信号传导受损。这是首次在丝状真菌中使SAP基因发生突变，并将SAP蛋白的功能与植物致病性和菌丝分支联系起来。
