Zhang Xiao-Feng, Wang Hai-Hua, Sun Xi-Yu, Pan Chun-Mei
Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China.
Henan Zhanggong old wine co.Ltd., Shangqiu, 476700, China.
Curr Microbiol. 2017 Dec;74(12):1389-1393. doi: 10.1007/s00284-017-1330-y. Epub 2017 Aug 24.
The Gram-stain-negative, rod-shaped and non-motile bacterial strain, designated ZGLJ7-1, was isolated from a pit mud. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain ZGLJ7-1 was related to the genus Lysobacter and had the highest 16S rRNA gene sequence similarity with the type strain of Lysobacter arseniciresistens ZS79 (97.4%). The predominant cellular fatty acids were iso-C, iso-Cω9c, iso-C and iso-C3-OH. Strain ZGLJ7-1 had Q-8 as the predominant ubiquinone. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, two unidentified aminolipids and two unidentified lipids. The genomic DNA G+C content of strain ZGLJ7-1 was 69.5 mol%. Strain ZGLJ7-1 shared DNA relatedness with 35% Lysobacter arseniciresistens CGMCC 1.10752. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that the strain ZGLJ7-1 is a representative of a novel species of the genus Lysobacter, for which we propose the name Lysobacter zhanggongensis sp. nov. (type strain ZGLJ7-1 = KACC 18547 = CGMCC 1.15404).
从窖泥中分离出一株革兰氏染色阴性、杆状且无运动性的细菌菌株，命名为ZGLJ7-1。基于16S rRNA基因序列的系统发育分析表明，菌株ZGLJ7-1与溶杆菌属相关，并且与抗砷溶杆菌ZS79模式菌株的16S rRNA基因序列相似度最高（97.4%）。主要的细胞脂肪酸为异-C、异-Cω9c、异-C和异-C3-OH。菌株ZGLJ7-1以Q-8作为主要的泛醌。极性脂质谱包含双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的磷脂、两种未鉴定的氨基脂质和两种未鉴定的脂质。菌株ZGLJ7-1的基因组DNA G+C含量为69.5 mol%。菌株ZGLJ7-1与抗砷溶杆菌CGMCC 1.10752的DNA相关性为35%。表型、系统发育和DNA-DNA相关性研究的综合数据表明，菌株ZGLJ7-1是溶杆菌属一个新物种的代表，我们将其命名为张氏溶杆菌（Lysobacter zhanggongensis sp. nov.）（模式菌株ZGLJ7-1 = KACC 18547 = CGMCC 1.15404）。
