Lysobacter zhanggongensis sp. nov. Isolated from a Pit Mud.

The Gram-stain-negative, rod-shaped and non-motile bacterial strain, designated ZGLJ7-1, was isolated from a pit mud. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain ZGLJ7-1 was related to the genus Lysobacter and had the highest 16S rRNA gene sequence similarity with the type strain of Lysobacter arseniciresistens ZS79 (97.4%). The predominant cellular fatty acids were iso-C, iso-Cω9c, iso-C and iso-C3-OH. Strain ZGLJ7-1 had Q-8 as the predominant ubiquinone. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, two unidentified aminolipids and two unidentified lipids. The genomic DNA G+C content of strain ZGLJ7-1 was 69.5 mol%. Strain ZGLJ7-1 shared DNA relatedness with 35% Lysobacter arseniciresistens CGMCC 1.10752. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that the strain ZGLJ7-1 is a representative of a novel species of the genus Lysobacter, for which we propose the name Lysobacter zhanggongensis sp. nov. (type strain ZGLJ7-1 = KACC 18547 = CGMCC 1.15404).