School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
Department of Bio and Environmental Technology, Seoul Women's University, Seoul, 01797, Republic of Korea.
Curr Microbiol. 2020 Jan;77(1):166-172. doi: 10.1007/s00284-019-01801-w. Epub 2019 Nov 14.
A Gram-negative, aerobic, motile by gliding, rod-shaped bacterium, strain 17J68-2, was isolated from a soil sample taken from Jeju Island, Republic of Korea. The isolate displayed high 16S rRNA gene sequence similarity to the members of the genus Lysobacter in the family Lysobacteraceae, with Lysobacter humi FJY8 (98.4% similarity), Lysobacter xinjiangensis RCML-52 (98.3%), and Lysobacter mobilis 9NM-14 (98.1%) as closest phylogenetic neighbors. Growth of strain 17J68-2 occurred at 15-42 °C, pH 7-8, and in the presence of 0-1.0% NaCl. Draft genome was 2.94 Mb in size with G+C content of 70.5 mol%. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylethanolamine. Ubiquinone Q-8 was the predominant respiratory quinone and the major fatty acids were C iso (39.4%), summed feature 3 (Cω7c/Cω6c) (6.6%), C iso 3-OH (6.4%), C iso (6.4%), and C iso H (6.2%). The DNA-DNA relatedness between strain 17J68-2 and L. humi, L. xinjiangensis, and L. mobilis were 39.9, 39.4, and 25.3%, respectively. From these results, it is concluded that the novel isolate possesses sufficient characteristics to differentiate it from the most closely affiliated Lysobacter species, and strain 17J68-2 represents a novel species of the genus Lysobacter, for which the name Lysobacter segetis sp. nov. (=KCTC 62237 = JCM 33058) is proposed.
一株革兰氏阴性、需氧、能滑行运动的杆状细菌，菌株 17J68-2，从韩国济州岛的土壤样本中分离得到。该分离株的 16S rRNA 基因序列与 Lysobacteraceae 家族中的 Lysobacter 属成员具有高度相似性，与 Lysobacter humi FJY8（98.4%相似性）、Lysobacter xinjiangensis RCML-52（98.3%）和 Lysobacter mobilis 9NM-14（98.1%）最为接近。菌株 17J68-2 的生长条件为 15-42°C、pH7-8 和 0-1.0%NaCl。基因组草图大小为 2.94Mb，GC 含量为 70.5mol%。主要极性脂为磷脂酰甘油、双磷脂酰甘油和磷脂酰乙醇胺。主要呼吸醌为泛醌 Q-8，主要脂肪酸为 C iso（39.4%）、总和特征 3（Cω7c/Cω6c）（6.6%）、C iso 3-OH（6.4%）、C iso（6.4%）和 C iso H（6.2%）。菌株 17J68-2 与 L. humi、L. xinjiangensis 和 L. mobilis 的 DNA-DNA 同源性分别为 39.9%、39.4%和 25.3%。由此可见，该新型分离株具有足够的特征可将其与最密切相关的 Lysobacter 种区分开来，菌株 17J68-2 代表 Lysobacter 属的一个新型种，命名为 Lysobacter segetis sp. nov.（=KCTC 62237=JCM 33058）。
