Stolz Andreas, Busse Hans-Jürgen, Kämpfer Peter
Institut für Mikrobiologie, Universität Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, Veterinärplatz 1, A-1210 Wien, Austria.
Int J Syst Evol Microbiol. 2007 Mar;57(Pt 3):572-576. doi: 10.1099/ijs.0.64761-0.
The taxonomic position of Pseudomonas sp. B13(T), isolated as a 3-chlorobenzoate-degrading organism and used for several groundbreaking studies on the enzymology and genetics of the degradative pathway for haloaromatic compounds, was studied in detail. The previously performed physiological studies, the detection of ubiquinone Q-9, the polyamine pattern with putrescine and spermidine as major polyamines, a fatty acid profile with C(18 : 1)omega7c, summed feature 3 and C(16 : 0) as quantitatively the most important constituents and the 16S rRNA gene sequence demonstrated that Pseudomonas sp. B13(T) indeed belongs to the genus Pseudomonas. The sequence of the Pseudomonas sp. B13(T) 16S rRNA gene demonstrated a high degree of similarity with that of Pseudomonas citronellolis DSM 50332(T) (98.9 %), Pseudomonas nitroreducens DSM 14399(T) (98.7 %), Pseudomonas jinjuensis DSM 16612(T) (98.1 %) and Pseudomonas multiresinivorans DSM 17553(T) (98.7 %). Thus it was shown that strain Pseudomonas sp. B13(T) can be distinguished from related species by the ability/inability to assimilate N-acetylgalactosamine, d-galactose, putrescine, trans-aconitate and mesaconate and some differences in the fatty acid profile. The positioning of Pseudomonas sp. B13(T) as a separate taxon was finally verified by DNA hybridization, which demonstrated less than 45 % DNA-DNA similarity between strain Pseudomonas sp. B13(T) and the reference strains. On the basis of these results, Pseudomonas sp. B13(T) represents a novel species for which the name Pseudomonas knackmussii sp. nov. is proposed. The type strain is B13(T) (=DSM 6978(T)=LMG 23759(T)).
对假单胞菌属菌株B13(T)的分类地位进行了详细研究。该菌株作为一种能降解3-氯苯甲酸的微生物被分离出来，并用于多项关于卤代芳烃化合物降解途径的酶学和遗传学的开创性研究。先前进行的生理学研究、泛醌Q-9的检测、以腐胺和亚精胺为主要多胺的多胺模式、以C(18 : 1)ω7c、总特征3和C(16 : 0)作为定量上最重要成分的脂肪酸谱以及16S rRNA基因序列表明，假单胞菌属菌株B13(T)确实属于假单胞菌属。假单胞菌属菌株B13(T)的16S rRNA基因序列与香茅假单胞菌DSM 50332(T)（98.9%）、硝基还原假单胞菌DSM 14399(T)（98.7%）、晋州假单胞菌DSM 16612(T)（98.1%）和多树脂假单胞菌DSM 17553(T)（98.7%）的序列具有高度相似性。因此表明，菌株假单胞菌属B13(T)可通过同化/不能同化N-乙酰半乳糖胺、D-半乳糖、腐胺、反式乌头酸和顺乌头酸的能力以及脂肪酸谱的一些差异与相关物种区分开来。假单胞菌属菌株B13(T)作为一个独立分类单元的定位最终通过DNA杂交得到验证，结果表明菌株假单胞菌属B13(T)与参考菌株之间的DNA-DNA相似性低于45%。基于这些结果，假单胞菌属菌株B13(T)代表一个新物种，为此提议将其命名为克纳克穆假单胞菌（Pseudomonas knackmussii sp. nov.）。模式菌株为B13(T)（=DSM 6978(T)=LMG 23759(T)）。
