Leibniz Institute of Photonic Technology , Jena 07745, Germany.
Friedrich Schiller University , Institute for Physical Chemistry, Jena 07743, Germany.
Anal Chem. 2017 Sep 19;89(18):9997-10003. doi: 10.1021/acs.analchem.7b02422. Epub 2017 Aug 25.
Deep UV resonance Raman spectroscopy is introduced as an analytical tool for ultrasensitive analysis of antibiotics used for empirical treatment of patients with sepsis and septic shock, that is, moxifloxacin, meropenem, and piperacillin in aqueous solution and human urine. By employing the resonant excitation wavelengths λ = 244 nm and λ = 257 nm, only a small sample volume and short acquisition times are needed. For a better characterization of the matrix urine, the main ingredients were investigated. The capability of detecting the antibiotics in clinically relevant concentrations in aqueous media (LODs: 13.0 ± 1.4 μM for moxifloxacin, 43.6 ± 10.7 μM for meropenem, and 7.1 ± 0.6 μM for piperacillin) and in urine (LODs: 36.6 ± 11.0 μM for moxifloxacin, and 114.8 ± 3.1 μM for piperacillin) points toward the potential of UV Raman spectroscopy as point-of-care method for therapeutic drug monitoring (TDM). This procedure enables physicians to achieve fast adequate dosing of antibiotics to improve the outcome of patients with sepsis.
深紫外共振拉曼光谱被引入作为一种分析工具，用于超灵敏分析用于经验性治疗脓毒症和脓毒性休克患者的抗生素，即莫西沙星、美罗培南和哌拉西林在水溶液和人尿中的分析。通过使用共振激发波长 λ = 244nm 和 λ = 257nm，仅需要小的样品体积和短的采集时间。为了更好地表征基质尿液，研究了主要成分。该方法在水相介质（LOD：莫西沙星为 13.0 ± 1.4μM，美罗培南为 43.6 ± 10.7μM，哌拉西林为 7.1 ± 0.6μM）和尿液（LOD：莫西沙星为 36.6 ± 11.0μM，哌拉西林为 114.8 ± 3.1μM）中检测到临床相关浓度的抗生素的能力表明，紫外拉曼光谱作为治疗药物监测（TDM）的即时检测方法具有潜力。该方法使医生能够快速给予足够剂量的抗生素，从而改善脓毒症患者的预后。
