Leibniz Institute of Photonic Technology, 07745 Jena, Germany.
Institute of Physical Chemistry, Friedrich Schiller University, 07743 Jena, Germany.
Molecules. 2019 Dec 10;24(24):4512. doi: 10.3390/molecules24244512.
Sepsis and septic shock exhibit a rapid course and a high fatality rate. Antibiotic treatment is time-critical and precise knowledge of the antibiotic concentration during the patients' treatment would allow individual dose adaption. Over- and underdosing will increase the antimicrobial efficacy and reduce toxicity. We demonstrated that fiber enhanced Raman spectroscopy (FERS) can be used to detect very low concentrations of ciprofloxacin in clinically relevant doses, down to 1.5 µM. Fiber enhancement was achieved in bandgap shifted photonic crystal fibers. The high linearity between the Raman signals and the drug concentrations allows a robust calibration for drug quantification. The needed sample volume was very low (0.58 µL) and an acquisition time of 30 s allowed the rapid monitoring of ciprofloxacin levels in a less invasive way than conventional techniques. These results demonstrate that FERS has a high potential for clinical in-situ monitoring of ciprofloxacin levels.
脓毒症和感染性休克表现出快速的病程和高死亡率。抗生素治疗时间紧迫，了解患者治疗过程中的抗生素浓度将允许进行个体化剂量调整。用药过量和不足都会增加抗菌效果并降低毒性。我们证明，纤维增强拉曼光谱（FERS）可用于检测临床相关剂量下非常低浓度的环丙沙星，低至 1.5µM。在带隙移动光子晶体光纤中实现了纤维增强。拉曼信号与药物浓度之间的高度线性关系允许对药物定量进行稳健的校准。所需的样品量非常低（0.58µL），采集时间为 30 秒，允许以比传统技术更微创的方式快速监测环丙沙星水平。这些结果表明，FERS 具有临床原位监测环丙沙星水平的巨大潜力。
