Priolo Vito, Masucci Marisa, Spada Eva, Proverbio Daniela, Pennisi Maria Grazia
1 Department of Veterinary Science, University of Messina, Polo Universitario Annunziata, Messina, Italy.
2 Veterinary Transfusion Research Laboratory (REVLab), Department of Veterinary Medicine, University of Milan, Milan, Italy.
J Feline Med Surg. 2018 Aug;20(8):690-695. doi: 10.1177/1098612X17727232. Epub 2017 Aug 25.
Objectives The aim of this study was to investigate the presence of naturally occurring antibodies against canine erythrocyte antigens in cats and vice versa. The influence of canine and feline blood type on cross-match results was also studied. Methods Blood samples from 34 cats and 42 dogs were used to perform test tube major and minor cross-match tests and blood typing. Blood from each cat was cross-matched with blood from 2-6 dogs, for a total of 111 cross-match tests. Haemolysis, macro- and microagglutination were considered markers of a positive cross-match. Results Eighty-three overall major cross-match tests were positive at 37°C, 86 at room temperature and 90 at 4°C. The minor cross-match tests were positive in all but two cross-matches performed at 37°C, all tests performed at room temperature and all but one test performed at 4°C. No cats tested totally negative at both major and minor cross-matches performed with samples from any single dog. Prevalence of warm natural antibodies against canine erythrocyte antigens was lower in type B cats than in type A cats, regardless of the blood type of donor dogs. Conclusions and relevance This study reveals a high prevalence of naturally occurring antibodies in cats against dog erythrocyte antigens and vice versa, and suggests that transfusion of cats with canine blood is not recommended as a routine procedure owing to the potential high risk of either acute severe or milder transfusion reactions.
目的 本研究旨在调查猫体内是否存在针对犬红细胞抗原的天然抗体，反之亦然。同时还研究了犬猫血型对交叉配血结果的影响。方法 采集34只猫和42只犬的血液样本，进行试管主次交叉配血试验和血型鉴定。每只猫的血液与2 - 6只犬的血液进行交叉配血，共进行111次交叉配血试验。溶血、宏观和微观凝集被视为交叉配血阳性的指标。结果 在37°C时，83次总体主交叉配血试验呈阳性，室温下为86次，4°C时为90次。次交叉配血试验中，除在37°C进行的两次交叉配血外均呈阳性，室温下所有试验均呈阳性，4°C时除一次试验外均呈阳性。在用任何一只犬的样本进行的主次交叉配血试验中，没有一只猫的检测结果完全为阴性。无论供血犬的血型如何，B型猫中针对犬红细胞抗原的温性天然抗体的发生率低于A型猫。结论及意义 本研究揭示猫体内存在针对犬红细胞抗原的天然抗体的高发生率，反之亦然，并表明由于存在急性严重或较轻输血反应的潜在高风险，不建议将犬血输给猫作为常规操作。
