Xia Fan, Chen Mei-Hong, Lv Ying-Ying, Zhang Han-Yun, Qiu Li-Hong
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3237-3245. doi: 10.1099/ijsem.0.002090. Epub 2017 Aug 25.
Three strains, DHOB09T, DHOC52T and DHON07T, were isolated from the forest soil of Dinghushan Biosphere Reserve, Guangdong Province, PR China. They were all Gram-stain-negative, aerobic, rod-shaped cells. The ranges (optimum) for the temperature, pH and NaCl concentration for growth of DHOB09T, DHOC52T and DHON07T were 10-42 (25-28) °C, pH 5.5-9.0 (7.0-7.5) and 0-4.0 (0-0.5) % (w/v); 10-42 (28) °C, pH 4.0-7.0 (4.5-6.5) and 0-2.0 (0) % (w/v) and 10-37 (25-28) °C, pH 4.0-7.5 (5.5-6.0) and 0-2.5 (0) % (w/v), respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that DHOB09T, DHOC52T and DHON07T formed a phyletic cluster with seven species of the genus Dyella within the major clade of Dyella with sequence similarities ranged from 96.9 to 98.6 %. This indicated that the three strains may represent three novel species of the genus Dyella. This result was also strongly supported by the concatenated analysis of partial gyrB, lepA and recA gene sequences. DNA-DNA hybridization between strains DHON07T and DHOB09T, as well as DHON07T and Dyella koreensis BB4T was much lower than 70 %. The G+C content of strains DHOB09T, DHOC52T and DHON07T were 59.4, 60.7 and 59.5 %, respectively. The major fatty acids of the three strains were iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 and the predominant respiratory lipoquinone was ubiquinone-8. All of the physiological, phylogenetic and chemotaxonomic data showed that strains DHOB09T, DHOC52T and DHON07T are distinctive from each other and from all species of the genus Dyellawith validly published names. Therefore, we suggest that they represent three novel species of the genus, for which the names Dyella caseinilytica sp. nov. (type strain DHOB09T=CGMCC 1.15434T=LMG 29202T), Dyella flava sp. nov. (type strain DHOC52T=NBRC 111979T=KCTC 52128T) and Dyella mobilis sp. nov. (type strain DHON07T=CGMCC 1.15400T=NBRC 111475T) are proposed.
从中国广东省鼎湖山生物圈保护区的森林土壤中分离出三株菌株，分别为DHOB09T、DHOC52T和DHON07T。它们均为革兰氏阴性、需氧、杆状细胞。DHOB09T、DHOC52T和DHON07T生长的温度范围（最适温度）、pH范围和NaCl浓度范围分别为10 - 42（25 - 28）℃、pH 5.5 - 9.0（7.0 - 7.5）和0 - 4.0（0 - 0.5）%（w/v）；10 - 42（28）℃、pH 4.0 - 7.0（4.5 - 6.5）和0 - 2.0（0）%（w/v）以及10 - 37（25 - 28）℃、pH 4.0 - 7.5（5.5 - 6.0）和0 - 2.5（0）%（w/v）。基于16S rRNA基因序列的系统发育分析表明，DHOB09T、DHOC52T和DHON07T在Dyella属的主要分支内与Dyella属的七个种形成一个系统发育簇，序列相似性范围为96.9%至98.6%。这表明这三株菌株可能代表Dyella属的三个新种。部分gyrB、lepA和recA基因序列的串联分析也有力地支持了这一结果。菌株DHON07T与DHOB09T以及DHON07T与韩国Dyella koreensis BB4T之间的DNA - DNA杂交率远低于70%。菌株DHOB09T、DHOC52T和DHON07T的G + C含量分别为59.4%、60.7%和59.5%。这三株菌株的主要脂肪酸为异C15 : 0、异C16 : 0和异C17 : 0，主要呼吸性脂醌为泛醌 - 8。所有的生理、系统发育和化学分类数据表明，菌株DHOB09T、DHOC52T和DHON07T彼此之间以及与所有已有效发表名称的Dyella属物种都不同。因此，我们建议它们代表该属的三个新种，分别命名为酪解Dyella caseinilytica sp. nov.（模式菌株DHOB09T = CGMCC 1.15434T = LMG 29202T）、黄色Dyella flava sp. nov.（模式菌株DHOC52T = NBRC 111979T = KCTC 52128T）和运动Dyella mobilis sp. nov.（模式菌株DHON07T = CGMCC 1.15400T = NBRC 111475T）。
